FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488628

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488628
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345286
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT19790.57314805697306No Hit
ACTATATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA18350.531443499012413No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13260.3840294712209589No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13130.3802644764050671No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10550.3055438100589077No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT10080.2919319057245298No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9270.2684730918716658No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8650.2505169627497205No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC8510.24646235294799096No Hit
ATATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT8450.24472466303296397No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTATA8150.23603621345782916No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG8150.23603621345782916No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG7890.22850622382604566No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT7860.2276373788685322No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7590.21981777425091084No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT7310.21170855464745167No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7010.20302010507231685No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6240.18071975116280417No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC6240.18071975116280417No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC6170.1786924462619394No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5730.165949386885075No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5350.15494401742323755No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5350.15494401742323755No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5290.1532063275082106No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC5000.1448074929189136No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4920.142490573032211No Hit
GGGTAAAGGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA4910.14220095804637314No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4910.14220095804637314No Hit
CTATATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT4900.14191134306053532No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4890.1416217280746975No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4850.1404632681313462No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA4730.13698788830129227No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4690.13582942835794093No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA4660.13496058340042746No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4520.13090597359869788No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG4480.1297475136553466No Hit
CTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCT4360.12627213382529265No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4360.12627213382529265No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4260.12337598396691438No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4160.1204798341085361No Hit
CCATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT4130.11961098915102263No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGGTTA4090.11845252920767131No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4050.11729406926432001No Hit
GTATTATCCAGAGTATATAGTTAATCATTACTTCCAGACGCGACATTATT3990.11555637934929305No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3930.1138186894342661No Hit
GTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA3910.11323945946259044No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA3890.11266022949091478No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA3850.11150176954756345No Hit
GGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3810.11034330960421215No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3750.1086056196891852No Hit
TTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGA3740.10831600470334737No Hit
ATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT3720.1077367747316717No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3680.1065783147883204No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC3650.10570946983080694No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3590.10397177991577995No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT3570.1033925499441043No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA3550.10281331997242865No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3540.10252370498659083No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG3520.10194447501491517No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT3520.10194447501491517No Hit
AGAGCATATAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTC3510.10165486002907734No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGAAGATCCAGC3470.10049640008572604No Hit
CTCTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC3470.10049640008572604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)