FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488629

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488629
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences345286
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT19980.5786507417039787No Hit
ACTATATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA13710.3970621455836611No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13200.3822917813059319No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG10100.29251113569620546No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9760.28266422617771936No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9750.2823746111918815No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9660.2797680763193411No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9580.27745115643263846No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8900.2577573373956662No Hit
ATATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTT8820.25544041750896357No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8340.2415388981887479No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7930.22966468376939697No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7820.22647891892518088No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7270.21055009470410035No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6950.2012824151572899No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6940.20099280017145207No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTATA6780.19635896039804684No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6000.1737689915026963No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5750.16652861685675063No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5640.16334285201253454No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5470.15841939725329146No Hit
ACACCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGG5390.15610247736658886No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG5340.15465440243739972No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT5280.15291671252237277No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5150.149151717706481No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA5030.1456763378764271No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA4960.14364903297556228No Hit
CTATATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT4950.14335941798972446No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4880.14133211308885968No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4810.13930480818799487No Hit
CCATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT4780.13843596323048138No Hit
GGGTAAAGGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA4750.13756711827296791No Hit
GTATTATCCAGAGTATATAGTTAATCATTACTTCCAGACGCGACATTATT4720.13669827331545442No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4690.13582942835794093No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG4680.13553981337210314No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4650.13467096841458964No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4550.13177481855621137No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4510.13061635861286006No Hit
CTTCAAAGACTGTGTGTTTAACGAGTGGGAGGAGTTGGGGGAGGAGGTTA4280.12395521393859003No Hit
ATCCAAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT4060.11758368425015785No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4040.11700445427848219No Hit
GTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4020.11642522430680655No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA3990.11555637934929305No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT3890.11266022949091478No Hit
CTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3870.11208099951923914No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT3860.1117913845334013No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG3800.11005369461837433No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG3760.10889523467502303No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT3720.1077367747316717No Hit
GGTTCAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA3660.10599908481664475No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA3660.10599908481664475No Hit
GTATATAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT3650.10570946983080694No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3640.10541985484496909No Hit
CTCTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC3640.10541985484496909No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3610.10455100988745562No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3530.102234090000753No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3480.10078601507156387No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAACCT3470.10049640008572604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)