FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488631

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488631
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences622793
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG45180.7254416796592126No Hit
CAATTGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT27600.44316490390868235No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT26320.4226123286549464No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG24590.39483423866356876No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT23410.37588733335153096No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG20040.32177625631630413No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATATTTGGTACGGTT19130.3071646598468512No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19120.3070040928526814No Hit
ATCCTAACCGTACCAAATATTTGCCTTTAGATAAAGGCATTAAACCTTAT18720.30058141308588887No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17360.2787443018787944No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT15200.24406183113811492No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT14800.2376391513713224No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCTTTAGATAAAGGCATTAAAC14590.23426724449375635No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13510.2169260091234166No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA13480.21644430814090715No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG13060.20970049438577507No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12910.20729198947322786No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12810.20568631953152972No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA12790.2053651855431901No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG12580.20199327866562405No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG12380.19878193878222783No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA12220.19621286687551082No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG11830.18995075410288811No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC11700.18786338317868057No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG11500.1846520432952843No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATATTTGGTA11320.1817618374002277No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11130.17871106451100124No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC11000.17662369358679367No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA10830.17389405468590688No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA10700.17180668376169933No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10470.1681136428957936No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC10300.1653840039949068No Hit
CAATGGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT10190.16361776705903888No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC10100.16217266411151057No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9950.1597641591989634No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9860.15831905625143505No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA9660.1551077163680388No Hit
ACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGCAAATA9390.1507724075254539No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9330.149809005560435No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC9170.147239933653718No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAGGC9000.1445102947528312No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8900.14290462481113309No Hit
CTCCCTCTTTTCCTCACATTCATTTGAAAGAGGATATTATCAATAGATGT8800.14129895486943495No Hit
ACCCCTCTCGGGGCCGTTTGGGGCTCTACCGTCCCCTTCTTCGTCTGCCG8770.1408172538869255No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC8390.13471570810847264No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTACTAAA8390.13471570810847264No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8340.13391287313762357No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA8220.13198606920758582No Hit
GACCCATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGGGCTTT8160.13102266724256695No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA8140.13070153325422734No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8130.1305409662600575No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC8090.12989869828337827No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7830.12572395643496312No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7800.1252422554524537No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7710.12379715250492539No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7640.12267318354573671No Hit
ACCCATCTCGGGGCCGTTTGGGGCTCTACCGTCCCCTTCTTCGTCTGCCG7590.12187034857488764No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA7210.11576880279643477No Hit
GTCTTTGGGTATACATTTAAACCCTACTAAAACTAAACGTTGGGGCTACT7150.1148054008314159No Hit
ACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAACTGTAAGA6990.1122363289246989No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6980.11207576193052908No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT6910.11095179297134039No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA6880.11047009198883095No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6880.11047009198883095No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6870.11030952499466115No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6780.10886442204713284No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6720.10790102008211397No Hit
GTTTAATGCCTTTATCTAAAGGCAAATATTTGGTACGGTTAGGATAGAAC6720.10790102008211397No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6710.10774045308794414No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6700.10757988609377434No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6700.10757988609377434No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC6680.1072587521054347No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG6590.1058136491579064No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAAG6530.10485024719288753No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG6520.1046896801987177No Hit
CCTATAAATAGACCAATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6410.10292344326284977No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT6390.10260230927451015No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG6370.10228117528617052No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC6330.10163890730949128No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6270.1006755053444724No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)