FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458726
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15860.3457401586132027No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT12790.2788156764604579No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA11360.24764238347074288No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8940.19488757994968675No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8780.1913996590557326No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8770.1911816639998605No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8510.18551379254718503No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8340.18180787659735878No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7530.16415027707171603No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7410.16153433640125042No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7130.1554304748368307No Hit
GTATAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7090.15455849461334217No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6780.14780064788130606No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6580.1434407467638634No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6560.14300475665211915No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6520.1421327764286306No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6500.14169678631688634No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6480.14126079620514206No Hit
CTCCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTT6440.14038881598165354No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6270.13668290003182726No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT6260.13646490497595515No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT6220.13559292475246662No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6010.13101502857915182No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5970.1301430483556633No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT5930.12927106813217476No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5840.12730911262932557No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5570.12142324612077798No Hit
CAATAGGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGT5550.12098725600903372No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5480.11946129061792879No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAG5360.11684534994746319No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC5350.11662735489159105No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5260.11466539938874186No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA5250.11444740433286973No Hit
GTTTTATACAGCAACAAGAGGGAAACATAGAGGTTCCTTGAGCAGGAATC5160.11248544883002054No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC5150.11226745377414842No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT5150.11226745377414842No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC5100.11117747849478773No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT5070.11052349332717135No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5050.11008750321542707No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT5020.10943351804781068No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4840.10550960704211229No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCGCAGGAACATATTGTACTAAAA4830.10529161198624015No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4820.10507361693036803No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4750.1035476515392631No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4720.10289366637164668No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4700.10245767625990242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)