FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences458726
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15750.3433422129986092No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT11170.24350047740917236No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10780.2349986702301592No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9450.20600532779916553No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG8980.19575956017317528No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8640.18834772827352275No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT8340.18180787659735878No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8240.17962792603863745No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGGCCCACAATTCTTTGACATACT8050.17548601997706692No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7810.17025413863613573No Hit
GTATAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC7500.16349629190409962No Hit
CTCCTAATCTCCTCCCCTAACTCCTCCCAGTCTTTAAACACACAGTCTTT7390.16109834628950614No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7370.1606623561777619No Hit
GTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAATA7120.15521247978095856No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6640.1447487170990962No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC6530.14235077148450273No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6430.1401708209257814No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6320.13777287531118795No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC6290.13711889014357156No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA6230.13581091980833876No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT6220.13559292475246662No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6070.13232299891438462No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5750.12534715712647637No Hit
GGTCTATTTACAGGCAATTTTCGAAAACATTGCTTGAGTTTTAGTACAAT5710.12447517690298784No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCTAACTCCTCCCAG5650.12316720656775505No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5650.12316720656775505No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5600.12207723128839437No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC5580.12164124117665012No Hit
GTTTTATACAGCAACAAGAGGGAAACATAGAGGTTCCTTGAGCAGGAATC5520.12033327084141732No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGCTACT5460.11902530050618451No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5400.11771733017095173No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5380.11728134005920744No Hit
GTTTTAGTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACA5310.11575537466810254No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5220.11379341916525335No Hit
ATATGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5040.10986950815955493No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA5020.10943351804781068No Hit
GTATAAAGAATTTGGAGCATCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4920.10725356748908935No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4890.10659958232147296No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4890.10659958232147296No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4830.10529161198624015No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4810.1048556218744959No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4780.10420163670687949No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4760.10376564659513522No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4710.10267567131577456No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4690.10223968120403029No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAATTGCCTGTAAATAGACCTAT4620.10071371581292536No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)