FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547926
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT25180.4595511072663097No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA20960.38253340779594325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20110.36702036406376043No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT13920.2540489044140997No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG13910.2538663980172505No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13240.24163846942835346No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12470.2275854768709643No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12220.2230228169497341No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12030.2195551954095991No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12010.21919018261590067No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11490.20969984997974178No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10230.1867040439767414No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC10200.18615652478619374No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG9770.17830874972167776No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9700.17703120494373328No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT9670.17648368575318563No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9340.17046097465716173No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT9330.1702784682603125No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9090.1658983147359315No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT9070.16553330194223306No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA8620.15732051408401865No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC8550.15604296930607417No Hit
CCTGTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA8440.15403539894073287No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8350.15239284136908998No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8110.14801268784470897No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT8030.14655263666991528No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7780.14198997674868505No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7780.14198997674868505No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC7660.13979989998649453No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA7640.13943488719279612No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7610.1388873680022485No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT7610.1388873680022485No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG7580.13833984881170086No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7450.13596726565266112No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT7370.13450721447786745No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7280.13286465690622457No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7180.13103959293773246No Hit
CTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT7180.13103959293773246No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG7120.1299445545566372No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA7010.1279369841912959No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6880.1255644010322562No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6790.1239218434606133No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT6770.12355683066691486No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6590.12027171552362911No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6500.11862915795198621No Hit
ACTACATGCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6490.11844665155513702No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGCATGTAGTTAATCATTAC6440.11753411957089097No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6370.11625657479294649No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA6330.11552654920554965No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6290.11479652361815282No Hit
CTCGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6260.11424900442760519No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC6150.11224143406226388No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6140.11205892766541468No Hit
CGTGTGGACTCTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAA6070.11078138288747019No Hit
GATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGG6040.11023386369692259No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6040.11023386369692259No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5990.10932133171267654No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5950.10859130612527969No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5900.10767877414103363No Hit
ATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA5840.10658373575993839No Hit
ATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA5780.10548869737884313No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5740.1047586717914463No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5700.10402864620404945No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5670.10348112701350182No Hit
GAGTTAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGA5570.10165606304500972No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5540.10110854385446211No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5540.10110854385446211No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)