FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386917
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT50041.2933006303677532No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT38540.9960792624774821No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA16160.41766063522667657No Hit
CGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTG14890.3848370580770553No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT13840.3576994549218566No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA13350.3450352401160972No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA11290.2917938472592313No Hit
GTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGT11210.28972622035216855No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10960.28326488626759744No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9770.252508936025039No Hit
CTCCTGAACATTGTTCACCTCACCATACGGCACTCAGGCAAGCTATTCTG9560.24708141539399922No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG9020.23312493377132562No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA8160.21089794452040103No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7940.20521197052597845No Hit
ATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCTTGAACTGGAGCC7830.20236898352876714No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT7720.19952599653155584No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7620.19694146289772743No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7390.190997035539922No Hit
CAATTCGTTGACATACTTTCCAATCAATGGGTCTGTTTACAGGAAGTTTC7240.18712023508917933No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7160.18505260818211658No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT7140.1845357014553509No Hit
TATCCACATTGTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG7110.18376034136520236No Hit
GAATGCAGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGA6920.1788497274609283No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAAGGGGCTGCA6820.17626519382709988No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6700.17316375346650573No Hit
GTTTCGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6430.16618551265516893No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG6400.1654101525650204No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT6260.1617918054776606No Hit
AGTCTAGACTCTGTGGTATTGTGTGGATTTTTGTCAACGAGAAAAACCCC6110.1579150050269179No Hit
CCTGTAAACAGACCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6110.1579150050269179No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA6080.15713964493676938No Hit
GGATATCCACATTGTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAAT6060.1566227382100037No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5960.15403820457617526No Hit
CATTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACCCC5920.15300439112264388No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5830.15067831085219827No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5730.14809377721836983No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT5610.14499233685777568No Hit
CGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTG5580.14421697676762715No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5520.1426662565873301No Hit
TAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATC5420.14008172295350166No Hit
CTTTTATCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGG5320.1374971893196732No Hit
CTTTTATCTTTGGGTATACATGTAAACCCTCACAAAACAAAAAGATGGGG5290.13672182922952467No Hit
GGTTTACATGTATACCCAAAGACACAAGAAAATTGGTAACAGCGGCATAA5240.13542956241261045No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5110.13206966868863348No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5110.13206966868863348No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT5090.1315527619618678No Hit
GTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5020.12974358841818787No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT4990.12896822832803934No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4970.12845132160127365No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4790.12379916106038245No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4790.12379916106038245No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4790.12379916106038245No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC4780.12354070769699961No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4770.12328225433361677No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATGGGTCTGT4760.12302380097023392No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG4730.1222484408800854No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4700.12147308078993685No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4670.12069772069978832No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4600.11888854715610842No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4550.1175962803391942No Hit
CAGCCGGTCTGGGGCAAAACTCATCGGGACTGACAATTCTGTCGTGCTCT4540.11733782697581135No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4480.11578710679551428No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT4470.11552865343213144No Hit
ATCAAACTGTGTTTTAGGAAACTTCCTGTAAACAGACCCATTGATTGGAA4450.11501174670536575No Hit
CCCTTAACTTCATGGGATATGTAATTGGGGGTTGGGGCACATTGCCACAG4450.11501174670536575No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4410.11397793325183436No Hit
CCCTTATTTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGC4390.11346102652506868No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4350.11242721307153732No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4300.11113494625462308No Hit
ATTCTTTGACATACTTTCCAATCAATGGGTCTGTTTACAGGAAGTTTCCT4300.11113494625462308No Hit
ACTACATGCTCTGGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATA4240.10958422607432602No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4200.10855041262079464No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4140.10699969244049756No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4120.10648278571373188No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4120.10648278571373188No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4090.10570742562358336No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4030.10415670544328628No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4030.10415670544328628No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC3980.10286443862637207No Hit
CTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3980.10286443862637207No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACGG3870.10002145162916076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)