FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386917
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT47411.2253273957980653No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT34060.8802921556819678No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT14410.3724312966346788No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA13690.353822654471114No Hit
CGTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTG13300.343742973299183No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA13210.34141689302873746No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA12470.32229134413840693No Hit
GTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGT11110.2871416867183401No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10990.284040246357746No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9890.2556103763856331No Hit
CTCCTGAACATTGTTCACCTCACCATACGGCACTCAGGCAAGCTATTCTG8790.2271805064135202No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT8790.2271805064135202No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8370.21632546515144074No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG8020.2072795974330412No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT7630.19719991626111028No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7370.19048012881315632No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACCCCCAATTACATATCCCATGAA7340.1897047687230078No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7240.18712023508917933No Hit
GTTTCGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6980.18040044764122537No Hit
ATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCTTGAACTGGAGCC6690.17290530010312288No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTTT6570.16980385974252876No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6550.16928695301576308No Hit
ACCTTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATT6440.16644396601855177No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT6390.16515169920163755No Hit
CAATTCGTTGACATACTTTCCAATCAATGGGTCTGTTTACAGGAAGTTTC6120.15817345839030075No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAAGGGGCTGCA6020.15558892475647232No Hit
TATCCACATTGTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG5900.15248748439587817No Hit
CATTTTGATTTTTTGTACAATATGTTCCTGTGGCAATGTGCCCCAACCCC5840.1509367642155811No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5770.1491275906719012No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5710.14757687049160415No Hit
GAATGCAGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGA5680.14680151040145561No Hit
GTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATT5540.14318316331409578No Hit
AGTCTAGACTCTGTGGTATTGTGTGGATTTTTGTCAACGAGAAAAACCCC5490.14189089649718156No Hit
CTTTTATCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGG5440.14059862968026735No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5390.13930636286335313No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5380.13904790949997028No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG5380.13904790949997028No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5340.1380140960464389No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5320.1374971893196732No Hit
GCCTAGCAGCCATGGGAACGATGTATACTTGCGGGAGAGCACGACAGAAT5300.1369802825929075No Hit
GGATATCCACATTGTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAAT5280.13646337586614182No Hit
CCTGTAAACAGACCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5250.1356880157759933No Hit
GTGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5150.13310348214216486No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5130.13258657541539917No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5130.13258657541539917No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG4990.12896822832803934No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT4960.1281928682378908No Hit
CGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTG4950.12793441487450796No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4930.1274175081477423No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4850.12534988124067953No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4850.12534988124067953No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT4850.12534988124067953No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC4820.124574521150531No Hit
GGTTTACATGTATACCCAAAGACACAAGAAAATTGGTAACAGCGGCATAA4800.1240576144237653No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA4780.12354070769699961No Hit
ATCAAACTGTGTTTTAGGAAACTTCCTGTAAACAGACCCATTGATTGGAA4690.121214627426554No Hit
ATGTAATTGGGGGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4650.12018081397302265No Hit
AGTATACATCGTTCCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4620.11940545388287412No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4560.11785473370257704No Hit
TAAACGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATCATC4490.11604556015889712No Hit
CTTTTATCTTTGGGTATACATGTAAACCCTCACAAAACAAAAAGATGGGG4420.1142363866152172No Hit
CCCTTATTTCAAAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGC4410.11397793325183436No Hit
ATTCTTTGACATACTTTCCAATCAATGGGTCTGTTTACAGGAAGTTTCCT4390.11346102652506868No Hit
CCCTTAACTTCATGGGATATGTAATTGGGGGTTGGGGCACATTGCCACAG4380.11320257316168585No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4370.112944119798303No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT4280.1106180395278574No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4240.10958422607432602No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACGG4140.10699969244049756No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4130.10674123907711472No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG4090.10570742562358336No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA4000.10338134535313775No Hit
CTTTCACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3940.10183062517284068No Hit
GACCAGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGGTTACGT3940.10183062517284068No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3890.10053835835592646No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC3880.10027990499254362No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)