FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481722
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC20420.42389593998198133No Hit
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG20160.4184986361428376No Hit
GTCTTTGATGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17620.3657711294065872No Hit
TCCCTATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA15990.33193418610733993No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15900.3300658886245594No Hit
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14310.29705929976210343No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC12390.2572022867961189No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12210.2534656918305579No Hit
CCTTCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9920.2059279003242534No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9510.1974167673471421No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9340.1938877609907789No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9290.19284981794478973No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9130.18952840019762435No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8800.18267797609409575No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8170.16959989371463208No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7970.16544812153067537No Hit
ATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA7510.1558990455075749No Hit
CCCTTACAGTCAACGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG7480.1552762796799814No Hit
ATATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7400.15361557080639873No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7380.15320039358800305No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT7350.15257762776040953No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT7310.15174727332361818No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7290.1513320961052225No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA7220.14987897584083767No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7200.149463798622442No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7030.14593479226607878No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACAAATATT6970.14468926061089177No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCTAACAATCCCCCATG6770.14053748842693503No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA6770.14053748842693503No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6570.13638571624297832No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG6020.12496834273709734No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG6010.12476075412789948No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6010.12476075412789948No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5950.12351522247271247No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG5930.1231000452543168No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5710.11853309585196442No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5700.11832550724276657No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5680.11791033002437089No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5610.11645720975998605No Hit
ATATTAACGTTGACATAGCTTACTACTAATTCCCTGGATGCTGGATCTTC5600.11624962115078823No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5570.11562685532319471No Hit
CCCTATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAG5510.11438132366800768No Hit
GAATTAGTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACA5460.11334338062201851No Hit
ATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT5440.11292820340362283No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5410.11230543757602934No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT5340.11085231731164447No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5330.11064472870244664No Hit
CTCTAAGGCCTCCCTATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5320.1104371400932488No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5280.10960678565645746No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5180.10753089956447912No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCCGTAAACACACAGTCTTTGA5150.10690813373688558No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5130.10649295651848992No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5130.10649295651848992No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5110.10607777930009424No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAACCGTAT5080.10545501347250075No Hit
GCACCGGACCATGCAAGACCTGCACAGCTCCTGCTCAAGGAACCTCTATG5080.10545501347250075No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5050.10483224764490723No Hit
TGGGCATACATTTGAACCCTAACAAAACAAAAAGATGGGGATATTCCCTT5030.10441707042651155No Hit
CACATAAGGTGGGCAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC4990.1035867159897202No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4970.10317153877132453No Hit
GCTTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC4910.10192600711613753No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG4870.10109565267934618No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC4860.10088806407014833No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4860.10088806407014833No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCTGTGGGCGTTCACGGTGGTCTC4860.10088806407014833No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT4830.10026529824255484No Hit
GACAAATATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACT4820.10005770963335699No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4820.10005770963335699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)