FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481722
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG21070.4373891995798407No Hit
CTCTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC19070.3958714777402734No Hit
GTCTTTGATGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16430.3410680849120447No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15800.32799000253258104No Hit
CTCTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT14220.29519100227932293No Hit
TCCCTATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA13910.28875575539419No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12500.25948576149729513No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC11050.2293854131636089No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9290.19284981794478973No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9260.1922270521171962No Hit
CCTTCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9020.18724492549644817No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8650.1795641469561282No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8600.17852620391013904No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8350.17333648868019313No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7830.16254188100190567No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7780.1615039379559165No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA7750.16088117212832298No Hit
ATATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7520.15610663411677272No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7370.15299280497880519No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7330.15216245054201386No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG7290.1513320961052225No Hit
ATATGTTCCTGTGGCAGTGTGCCCCAACTCCCAATTACATATCCCATGAA7200.149463798622442No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7060.14655755809367227No Hit
CCCTTACAGTCAACGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG7040.1461423808752766No Hit
ATGCAGAAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGA6990.14510443782928742No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACAAATATT6690.13887677955335237No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT6660.13825401372575882No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT6540.1357629504153848No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG6540.1357629504153848No Hit
CGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCT6520.13534777319698915No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6480.1345174187601978No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG5990.12434557690950382No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCTAACAATCCCCCATG5950.12351522247271247No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5840.12123174777153628No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5790.12019380472554711No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5710.11853309585196442No Hit
ATATTAACGTTGACATAGCTTACTACTAATTCCCTGGATGCTGGATCTTC5690.11811791863356874No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC5650.1172875641967774No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5590.11604203254159037No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5450.11313579201282066No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5370.111475083139238No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC5350.11105990592084232No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5280.10960678565645746No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5260.10919160843806178No Hit
GAATTAGTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAACTCAGACA5220.10836125400127043No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5170.10732331095528126No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5100.10587019069089641No Hit
TGGGCATACATTTGAACCCTAACAAAACAAAAAGATGGGGATATTCCCTT5090.10566260208169857No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5090.10566260208169857No Hit
CACATAAGGTGGGCAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5070.1052474248633029No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT4890.10151082989774186No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4870.10109565267934618No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4870.10109565267934618No Hit
GCTTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC4860.10088806407014833No Hit
ATAAGAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT4850.10068047546095052No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)