FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401117
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24960.6222623324366706No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21980.5479697943492796No Hit
CTCATTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA17010.4240657962639329No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT15480.38592231194389665No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA14260.3555072460155017No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13670.34079832068947463No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13270.33082616792606645No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT12320.307142305112972No Hit
CTTTAGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGGA10520.26226761767763523No Hit
CAATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG10050.2505503381806306No Hit
CTCCAAAGGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA9400.2343455899400923No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8930.22262831044308767No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT8420.20991381566974224No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTATATATCCCATGAA8390.20916590421248663No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8320.2074207774788902No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA8070.20118818200176009No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7930.19769792853456722No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCCAGA7740.19296115597194832No Hit
GCTTTAGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGG7670.1912160292383519No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT7530.18772577577115904No Hit
ATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA7490.18672856049481823No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTCGGCGA7320.18249039557036975No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7200.17949874974134727No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA7150.17825223064592127No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT7050.1757591924550692No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTCGGCGAGAAAGAAA6860.17102241989245034No Hit
CATTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT6790.1692772931588539No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6780.1690279893397687No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT6760.16852938170159829No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6490.16179817858629778No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6420.16005305185270133No Hit
CATGTATACAAGCAAAACAGGCTTTTTCTTTCTCGCCGACTTATAAGGCC6320.1575600136618493No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6170.1538204563755712No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6080.15157672200380437No Hit
ATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG6070.15132741818471918No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5870.14634134180301508No Hit
GTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5800.14459621506941867No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC5780.14409760743124825No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5620.14010874632588496No Hit
GCTTTGGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGG5540.13811431577320332No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT5500.13711710049686252No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5350.13337754321058445No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACCAAACC5350.13337754321058445No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5330.13287893557241404No Hit
CAGTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5320.13262963175332884No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTTCTAAAAC5270.13138311265790278No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5130.12789285919070995No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5120.12764355537162472No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA5110.12739425155253953No Hit
CTCTAAAGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5080.12664634009528392No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA4990.12440260572351709No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4990.12440260572351709No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4980.12415330190443188No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTGTTATGC4980.12415330190443188No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTTGATCTTC4910.12240817517083544No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC4880.12166026371357984No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4860.12116165607540942No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTGTTATGCAAGACCTTGGG4860.12116165607540942No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT4860.12116165607540942No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4800.1196658331608982No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4700.11717279497004615No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT4680.11667418733187573No Hit
TGGAAACCACCGTGAACGCCCACCGGAACTTGCCCAAGGTCTTGCATAAC4670.11642488351279053No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC4630.11542766823644972No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC4590.1144304529601089No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTTCTTT4540.11318393386468288No Hit
CTTCAAAGACAGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA4520.11268532622651246No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTACTCTTCTACGGTACCTTGC4460.11118950331200123No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4460.11118950331200123No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT4420.11019228803566042No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTCGGCGAGAAAGAA4420.11019228803566042No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4350.108447161302064No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAAGGGGCGGCA4340.10819785748297879No Hit
CCCTTAACTTCATGGGATATATAATTGGGAGTTGGGGCACATTGCCACAG4320.10769924984480837No Hit
CTGTTTACTTAGAAAGGCCTTATAAGTCGGCGAGAAAGAAAAAGCCTGTT4310.10744994602572316No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT4280.10670203456846755No Hit
TATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4270.10645273074938236No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4250.10595412311121194No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC4200.10470760401578591No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4190.1044583001967007No Hit
GCTCTGTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCA4180.10420899637761552No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4170.10395969255853031No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4160.1037103887394451No Hit
TATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAG4160.1037103887394451No Hit
AGTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATA4150.1034610849203599No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4120.10271317346310428No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4100.10221456582493386No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4090.10196526200584868No Hit
GCACAACTTCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACC4070.10146665436767827No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4060.10121735054859306No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT4050.10096804672950785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)