FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401117
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24810.6185227751503926No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC20330.5068346642002208No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT15320.3819334508385334No Hit
CTCATTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA13440.3350643328505149No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13010.32434426862985116No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA12510.31187907767559087No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11120.2772258468227475No Hit
CTCCAAAGGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA10800.2692481246120209No Hit
CTTTAGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGGA10330.25753084511501634No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10040.2503010343615454No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT9590.2390823625027112No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8610.21465058823236113No Hit
ATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA8400.20941520803157182No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTATATATCCCATGAA8200.20442913164986773No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA8080.20143748582084528No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7420.1849834337612218No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7420.1849834337612218No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTCGGCGA7410.1847341299421366No Hit
GCTTTAGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGG7330.18273969938945495No Hit
GTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGTACAATATGTT7110.17725501536958044No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7070.17625780009323963No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7030.17526058481689882No Hit
CAATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG7020.1750112809978136No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGAAAACTTCCTGTAA6780.1690279893397687No Hit
CCCTTACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCCAGA6750.1682800778825131No Hit
CAATAGGCCTGTTTACAGGAAGTTTTCTAAAACACATTTTGATTTTTTGT6740.16803077406342787No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6680.16653495114891664No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6660.16603634351074625No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6430.16030235567178655No Hit
GTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG6320.1575600136618493No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTCGGCGAGAAAGAAA6300.15706140602367888No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6170.1538204563755712No Hit
CTTCAAAGACAGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATTA5990.14933298763203753No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5830.14534412652667425No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5450.13587058140143649No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5420.13512266994418087No Hit
GCTTTGGGGCATGGACATTGACCACTATAAAGAATTTGGAGCTTCTGTGG5400.13462406230601046No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5290.1318817202960732No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAA5270.13138311265790278No Hit
CGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCCT5250.1308845050197324No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5210.1298872897433916No Hit
CTCTAAAGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5200.12963798592430636No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC5170.12889007446705078No Hit
CACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCT5160.12864077064796556No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5110.12739425155253953No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5060.12614773245711353No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4980.12415330190443188No Hit
ATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG4950.12340539044717626No Hit
CATTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4900.12215887135175024No Hit
ACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACCAAACC4900.12215887135175024No Hit
CATGTATACAAGCAAAACAGGCTTTTTCTTTCTCGCCGACTTATAAGGCC4800.1196658331608982No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTTGATCTTC4780.11916722552272778No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA4760.11866861788455738No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTCGGCGAGAAAGAA4710.11742209878913136No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4640.11567697205553491No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAGGACACCAAATATTCAAGAA4630.11542766823644972No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT4620.11517836441736452No Hit
GTAAACACACTGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4570.11393184532193848No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC4530.11293463004559767No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT4340.10819785748297879No Hit
CCCTTAACTTCATGGGATATATAATTGGGAGTTGGGGCACATTGCCACAG4320.10769924984480837No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4320.10769924984480837No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4310.10744994602572316No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4290.10695133838755275No Hit
GCTTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4280.10670203456846755No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4230.10545551547304154No Hit
ATATATAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAA4230.10545551547304154No Hit
GCTCTGTATCGGGAGGCTTTAGAGTCTCCGGAACATTGTTCACCTCACCA4160.1037103887394451No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTTCTTT4140.10321178110127469No Hit
CTGTTTACTTAGAAAGGCCTTATAAGTCGGCGAGAAAGAAAAAGCCTGTT4140.10321178110127469No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4100.10221456582493386No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4080.10171595818676347No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)