FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445877
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG33130.7430300284607638No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG19130.42904209008313954No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15680.35166649098293923No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT15320.34359251542465746No Hit
CATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT13730.307932456708913No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT13520.30322263763324864No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13040.29245733688887293No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT12170.272945229289692No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA11880.26644119342329836No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG10240.22965974921334809No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10060.2256227614342072No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG9970.22360426754463675No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA9880.22158577365506632No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9820.22024011106201938No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9790.21956727976549587No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC9770.21911872556781356No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9530.21373607519562568No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9380.2103719187130083No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT9080.2036436057477735No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9060.20319505155009118No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA8880.1991580637709503No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA8810.19758812407906215No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8780.1969152927825387No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT8670.19444824469528593No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8630.19355113629992127No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA8620.19332685920108011No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8380.18794420882889226No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8340.18704710043352762No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8030.18009451036945168No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7830.17560896839262846No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC7740.173590474503058No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA7720.1731419203053757No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC7420.1664136073401409No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC7310.16394655925288812No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7160.16058240277027072No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC7050.15811535468301796No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6690.1500413791247362No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA6690.1500413791247362No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6680.14981710202589504No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAA6660.1493685478282127No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6510.1460043913455953No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6320.14174312646761325No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6160.1381546928861547No Hit
ATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTA6150.13793041578731355No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6130.13748186158963122No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6060.1359119218977431No Hit
GTATACATCTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAAC6040.1354633677000608No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6010.1347905364035373No Hit
CAATAGGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT5920.13277204251396685No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5920.13277204251396685No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5780.1296321631301906No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5650.12671656084525554No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG5560.12469806695568508No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA5530.12402523565916161No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5460.12245529596727348No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG5460.12245529596727348No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5340.11976397078117956No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG5330.11953969368233841No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5220.11707264559508565No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCT5170.11595126010087985No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG5150.11550270590319751No Hit
GAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTTCCTGTGGT5110.11460559750783289No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5100.11438132040899171No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5080.1139327662113094No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5050.11325993491478592No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5040.11303565781594475No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAGATGTATA4970.11146571812405665No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4960.11124144102521547No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC4890.10967150133332736No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT4850.1087743929379627No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4840.10855011583912155No Hit
GCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAG4800.10765300744375692No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAAC4760.10675589904839226No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTTTCGCCAACTTACAAGGCC4740.10630734485070994No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4740.10630734485070994No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4720.10585879065302764No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4670.10473740515882182No Hit
GGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAA4660.10451312805998067No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4630.10384029676345717No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT4620.10361601966461602No Hit
AGGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAAT4580.1027189112692514No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA4560.10227035707156906No Hit
CAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATT4490.10070041737968094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)