FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481132
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21240.44145889277786554No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG17750.36892162649751004No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT15130.314466715994779No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC14430.29991769410473634No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13330.27705494542038356No Hit
ATTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT13330.27705494542038356No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC13090.27206670934379756No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT12940.26894906179593125No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG12890.2679098459466425No Hit
ATATGTTCCTGTGGCAATGAGCCCCAACTCCCAATTACATATCCCATGAA12150.2525294513771688No Hit
GTTGTGAATATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCTCCCATG12100.2514902355278801No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT11420.23735689997755294No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11400.23694121363783743No Hit
CCCTAAAAGACCTACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT10980.22821180050381185No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA10000.20784316985775214No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT9690.2014000315921618No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9250.19225493211842073No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT8840.18373336215425287No Hit
TATCCACATTGTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCG8820.1833176758145374No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8720.18123924411595985No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8630.17936865558724008No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8600.17874512607766682No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA8490.17645885120923158No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8490.17645885120923158No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT8470.17604316486951604No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT8460.17583532169965832No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC8460.17583532169965832No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG8430.17521179219008504No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACGG8370.17396473317093855No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG8170.16980786977378348No Hit
GGATATCCACATTGTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAAT8030.16689806539577498No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAAGGGGCAGCG7990.16606669271634397No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGA7780.16170198614933115No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG7780.16170198614933115No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACGGCACTCAGGCA7390.15359610252487882No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7370.15318041618516334No Hit
ATGTAATTGGGAGTTGGGGCTCATTGCCACAGGAACATATTGTACAAAAA7260.15089414131672804No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTACCTAAC7150.14860786644829277No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG7060.146737277919573No Hit
TCCACATTGTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCGTT7010.14569806207028424No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6990.14528237573056874No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6970.14486668939085323No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAA6890.14320394403199121No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC6890.14320394403199121No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6840.14216472818270245No Hit
GCTTTATTCTTCTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT6830.1419568850128447No Hit
GGCTTTCAGTTATATGGATGATATGGTATTGGGGGCCAAGTCTGTACAAC6750.1402941396539827No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA6720.13967061014440943No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG6710.1394627669745517No Hit
GTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCGTTGACATACT6680.13883923746497842No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6660.13842355112526292No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATTCAAGCTAAACAG6650.13821570795540516No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA6650.13821570795540516No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC6640.1380078647855474No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACGG6590.13696864893625865No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT6570.13655296259654315No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6460.13426668772810788No Hit
GTAGAGGCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG6420.1334353150486769No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6350.1319804128596726No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCA6300.13094119701038384No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6240.12969413799123733No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTA6230.12948629482137958No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6230.12948629482137958No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6130.12740786312280206No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATTCAAGCTAAAC6070.12616080410365554No Hit
ACTAAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG6040.1255372745940823No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGGTTT6000.12470590191465128No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5920.12304315655578925No Hit
CTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTT5920.12304315655578925No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC5900.12262747021607376No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5890.12241962704621602No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5800.12054903851749625No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5750.11950982266820748No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5720.11888629315863423No Hit
TCCTAATACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT5700.11847060681891872No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT5690.11826276364906096No Hit
TTCTTCTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC5640.11722354779977222No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5620.1168078614600567No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5570.11576864561076794No Hit
GGTTGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGTC5560.11556080244091019No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCA5550.11535295927105242No Hit
GAATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA5500.11431374342176367No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5470.11369021391219043No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5430.11285884123275942No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG5360.11140393904375515No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5350.1111960958738974No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTAATCTCCTCC5340.11098825270403963No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5290.10994903685475088No Hit
GCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTTTAGCTTGAATACATGC5240.10890982100546212No Hit
GTACAATATGTTCCTGTGGCAATGAGCCCCAACTCCCAATTACATATCCC5240.10890982100546212No Hit
GGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTA5210.10828629149588886No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5200.10807844832603111No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT5170.10745491881645784No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5160.1072470756466001No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5140.10683138930688461No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5110.10620785979731134No Hit
CTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGAC5030.10454511443844933No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT5010.10412942809873381No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5000.10392158492887607No Hit
GTAAAAGCCTGTTTAGCTTGAATACATGCATATAAAGGCATTAAAGCAGG4990.10371374175901832No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCATTGCCACAG4990.10371374175901832No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4970.1032980554193028No Hit
CCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCATGTAGTTAATCA4890.10163531006044078No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4880.10142746689058303No Hit
ACTACATGCTCTGGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAGTA4880.10142746689058303No Hit
GTATGGATCGGCAAAGGAGACACAAAGGTTCCACGCATGCGCCGATGGCC4870.1012196237207253No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4840.10059609421115202No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4840.10059609421115202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)