FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481132
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14850.30864710723876193No Hit
CATTTAAACCCTCATAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT14730.30615298920046885No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC14450.30033338044445185No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13880.28848631976255995No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG13790.2866157312338402No Hit
GGATAATAAGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT11930.24795690164029827No Hit
ATTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT11760.24442356775271648No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10910.22675689831480758No Hit
ATATGTTCCTGTGGCAATGAGCCCCAACTCCCAATTACATATCCCATGAA10870.22592552563537657No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT10830.22509415295594556No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC10700.2223921917477948No Hit
GGAACATATTGTACAAAAAATCAAATCATGTTTTAGGAAACTTCCTGTAA10680.22197650540807926No Hit
GTTGTGAATATGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCTCCCATG10480.21781964201092424No Hit
CCCTAAAAGACCTACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT9940.2065961108386056No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9560.19869807038401105No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG9060.18830591189112342No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT8640.17957649875709783No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA8460.17583532169965832No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8390.17438041951065403No Hit
GTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGTACAATATGTT8120.16876865392449475No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT8080.16793728124506374No Hit
ATGTAATTGGGAGTTGGGGCTCATTGCCACAGGAACATATTGTACAAAAA7930.16481963369719743No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACGG7860.16336473150819317No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT7800.16211767248904665No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7670.15941571128089588No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC7670.15941571128089588No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA7560.1571294364124606No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG7440.15463531837416758No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7360.15297257301530556No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC7290.15151767082630133No Hit
GCTTTATTCTTCTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT6990.14528237573056874No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTAGGTCT6840.14216472818270245No Hit
GCCTTAGAGTCTCCGGAACATTGCTCACCTCACCATACGGCACTCAGGCA6820.14174904184298695No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6770.1407098259936982No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATGATTTGATTTTTTGT6620.1375921784458319No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG6450.13405884455825012No Hit
GGCTTTCAGTTATATGGATGATATGGTATTGGGGGCCAAGTCTGTACAAC6380.13260394236924586No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATTCAAGCTAAACAG6330.1315647265199571No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTACCTAAC6210.12907060848166407No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATTCAAGCTAAAC6140.1276157062926598No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA6060.1259529609337978No Hit
TATCCACATTGTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCG6060.1259529609337978No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT6040.1255372745940823No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6040.1255372745940823No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6020.12512158825436678No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5970.12408237240507804No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5950.12366668606536252No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5920.12304315655578925No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5870.1220039407065005No Hit
CCCTTAATTTCATGGGATATGTAATTGGGAGTTGGGGCTCATTGCCACAG5860.12179609753664275No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAAGGGGCAGCG5810.12075688168735399No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGAGAAAGTAA5770.11992550900792298No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5750.11950982266820748No Hit
GGCCTCTACCGCCCGCTTCTCCGTCTGCCGTACCGACCGACCACGGGGCG5740.11930197949834973No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC5730.11909413632849197No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5660.11763923413948771No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG5600.1163921751203412No Hit
GGATATCCACATTGTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAAT5550.11535295927105242No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5460.11348237074233267No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5460.11348237074233267No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5400.11223531172318615No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTATAAGTTGGCGA5400.11223531172318615No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT5400.11223531172318615No Hit
GTGTGAAAGGGGCAGCGAACCCTAAAAGACCTACAATTCGTTGACATACT5350.1111960958738974No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCTTATTATCCAGAGCA5320.11057256636432414No Hit
GTAATTGGGAGTTGGGGCTCATTGCCACAGGAACATATTGTACAAAAAAT5320.11057256636432414No Hit
GGTTGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGTC5280.10974119368489313No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5270.10953335051503538No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5240.10890982100546212No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA5230.10870197783560437No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5210.10828629149588886No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTA5150.10703923247674235No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5120.1064157029671691No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5120.1064157029671691No Hit
TTCTTCTTCTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC5100.10600001662745359No Hit
GTAGAGGCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG5050.10496080077816482No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5040.10475295760830708No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG5040.10475295760830708No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGCTCACCTCACCA5020.10433727126859157No Hit
GCTTTAATGCCTTTATATGCATGTATTCAAGCTAAACAGGCTTTTACTTT4990.10371374175901832No Hit
ATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATAAGGTTT4950.10288236907958731No Hit
ATGTAAGACCTTGGGCAGGTTCCGGTGGGCGTTCACGGTGGTCTCCATGC4930.10246668273987179No Hit
ACTAAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG4890.10163531006044078No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4850.10080393738100978No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)