FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528191
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52720.9981237847672528No Hit
ATCCTAACTGTACCAAATATTTGCCCCTAGATAAAGGCATTAAACCTTAT13770.2607011478802176No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13560.256725313380955No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG9870.18686422146534112No Hit
GGATATTGTCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG9820.18591759420361195No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9380.17758727430039511No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA8660.16395584173149486No Hit
GTTCTATCCTAACTGTACCAAATATTTGCCCCTAGATAAAGGCATTAAAC8480.16054798358926978No Hit
AGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA8400.1590333799705031No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8360.15827607816111974No Hit
GATTAACTATGTGTTCAGGGTAATAAGGTTTAATGCCTTTATCTAGGGGC8080.1529749654954363No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT7890.1493777819008654No Hit
GTAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTGGTACAGTTAG7670.145212621949257No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG7660.14502329649691117No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6860.1298772603092442No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6820.12911995849986085No Hit
GTCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAG6630.12552277490528993No Hit
GGGTAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTGGTACAGTT6500.12306154402479405No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC6370.12060031314429817No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGG6290.11908570952553149No Hit
GATTGACTATGTGTTCAGGGTAATAAGGTTTAATGCCTTTATCTAGGGGC5850.11075538962231465No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5810.10999808781293131No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGACAATATCCTCTTTC5650.10696888057539793No Hit
TTCTTCTACTGTACCTGTCTTCAATCCTGACTGGCAAACTCCCTCTTTTC5500.10412899879021036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)