FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528191
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN52510.9941479502679903No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG12950.24517646078785893No Hit
ATCCTAACTGTACCAAATATTTGCCCCTAGATAAAGGCATTAAACCTTAT12490.23646748997995043No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG10700.2025782340100456No Hit
GGATATTGTCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATG10480.19841307405843717No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9380.17758727430039511No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGA8720.16509179444556987No Hit
GTTCTATCCTAACTGTACCAAATATTTGCCCCTAGATAAAGGCATTAAAC8420.15941203087519476No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG8420.15941203087519476No Hit
AGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA8100.15335361640012798No Hit
GATTAACTATGTGTTCAGGGTAATAAGGTTTAATGCCTTTATCTAGGGGC7700.1457805983062945No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT7420.14047948564061108No Hit
GTAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTGGTACAGTTAG7120.13479972207023594No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6800.1287413075951692No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6750.12779468033344No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTGACTATGTGTTCAGG6670.12628007671467328No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT6500.12306154402479405No Hit
GTCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAG6460.12230424221541071No Hit
GGGTAATAAGGTTTAATGCCTTTATCTAGGGGCAAATATTTGGTACAGTT6040.11435257321688555No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCATTAAC5800.10980876236058547No Hit
CTACTGTACCTGTCTTCAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5590.10583292786132288No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT5510.10431832424255619No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)