FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598957
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG40590.6776780303093545No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26640.44477316401678246No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT25250.4215661558342252No Hit
CTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGG20430.3410929332155731No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG17050.2846615032464768No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCG16440.2744771327490955No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT15630.2609536243837204No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGA14630.24425793504375104No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC14580.24342315057675257No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14580.24342315057675257No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG13430.22422310783578786No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12820.2140387373384066No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA12820.2140387373384066No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCGTTAAC12570.20986481500341425No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA12390.20685959092221978No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12360.20635872024202073No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTATG12180.20335349616082624No Hit
AGGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAAC10360.1729673415620821No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT10350.1728003846686824No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT10300.17196560020168394No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAGAA10160.16962820369408824No Hit
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT9910.1654542813590959No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9560.15961079009010665No Hit
GGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG9540.15927687630330725No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGA9480.1582751349429091No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9420.1572733935825109No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA9140.15259860056731953No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT9070.15142990231352169No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9010.1504281609531235No Hit
TTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAGA8840.14758989376532872No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC8810.14708902308512964No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT8600.1435829283237361No Hit
GTTTGATGCTGTATCTCTTGTTCCCAAGAATATGGTGACCCAAAAAATGA8560.14291510075013733No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC8170.1364037819075493No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8020.1338994285065539No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG7720.1288907217045631No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7700.1285568079177637No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT7700.1285568079177637No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT7680.12822289413096433No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7670.12805593723756462No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA7660.12788898034416493No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7430.12404897179597199No Hit
GAGGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAA7340.12254635975537476No Hit
AAGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTAT7290.12171157528837628No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7260.1212107046081772No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTA7150.11937417878078059No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7140.11920722188738091No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG7120.11887330810058151No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6900.11520025644578827No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA6850.1143654719787898No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6800.11353068751179132No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT6700.1118611185777944No Hit
TAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTCA6470.10802111002960145No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6420.10718632556260298No Hit
CCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG6370.10635154109560453No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC6350.10601762730880514No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6270.1046819721616076No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6260.10451501526820792No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6150.10267848944081126No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6140.10251153254741158No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAG6120.10217761876061218No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6050.10100892050681434No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6050.10100892050681434No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6000.10017413603981588No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5990.1000071791464162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)