FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488649

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488649
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences598957
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG45080.7526416754458166No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN26570.4436044657629846No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT23550.3931834839562773No Hit
CTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTATGCCTGCTAGG19960.3332459592257875No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT16270.27163886556130074No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG15770.26329102089131606No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCG15500.25878318476952433No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT14760.2464283746579471No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGA14050.23457443522656887No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC13890.23190312493217377No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13840.2310683404651753No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA13690.2285639870641799No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG13210.22055005618099463No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA12630.21086655636381244No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTATG12290.20519002198822284No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTCTTTCGTTAAC11950.1995134876126333No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11930.19917957382583393No Hit
ATTTATAGGAAGTTTCCGAAAGCATTGTTTGATTTTATGTACAATATGTT9930.16578819514589527No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC9760.1629499279581005No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTCACTTCAT9710.16211514349110204No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCTTTGACATACT9590.16011166077030572No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC9550.15944383319670694No Hit
GTTTGATGCTGTATCTCTTGTTCCCAAGAATATGGTGACCCAAAAAATGA9370.15643860911551247No Hit
ATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAGGCATAA9230.15410121260791676No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9140.15259860056731953No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAGAA8970.14976033337952474No Hit
TTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAGA8940.14925946269932566No Hit
AGGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAAC8890.1484246782323272No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGA8640.14425075589733488No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT8630.14408379900393517No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC8620.1439168421105355No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8610.1437498852171358No Hit
GGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8580.14324901453693673No Hit
GTAGTAGGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT8500.14191335938973917No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC8320.13890813530854468No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG7780.12989246306496124No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7730.12905767859796277No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7720.1288907217045631No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7650.12772202345076525No Hit
CCCTTCACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG7460.12454984247617108No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7330.12237940286197507No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7260.1212107046081772No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7250.12104374771477752No Hit
GATTAAAATTGATTATGCCTGCTAGGTTCTATCCTAACCGTACCAAATAT7220.12054287703457844No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT7120.11887330810058151No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC6990.11670286848638549No Hit
ATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT6840.11419851508539011No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6830.1140315581919904No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAGAAGGAGATTAAAATTGATTA6770.11302981683159224No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT6690.1116941616843947No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6630.11069242032399655No Hit
GAGGACATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAA6500.10852198070980053No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG6480.10818806692300116No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6470.10802111002960145No Hit
CTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6460.10785415313620177No Hit
GCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAACCTAGCAG6400.10685241177580361No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA6390.10668545488240391No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTTAGGATAGAAC6360.10618458420220483No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6330.10568371352200576No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6310.10534979973520636No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6290.105015885948407No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6290.105015885948407No Hit
AAGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTAT6290.105015885948407No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6270.1046819721616076No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6180.10317936012101037No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAG6060.10117587740021404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)