FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589258
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN92451.5689222717383557No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG42540.7219248614359075No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT33680.5715662748745032No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG21530.365374759443232No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG21030.35688951189462No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG20090.3409372465032295No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT17690.300208058269892No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG16780.28476490773141816No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA15970.2710188067026667No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT15920.27017028194780557No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14960.25387860665447054No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC14090.23911427591988568No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA13930.23639899670432987No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT13830.23470194719460746No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA13810.234362537292663No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG12710.21569499268571662No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT12360.20975531940168823No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA11820.2005912520491873No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT11700.19855479263752043No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT10970.1861663312165469No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10720.18192370744224093No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA10670.18107518268737974No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG10570.17937813317765733No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC10430.17700226386404597No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC10390.17632344406015701No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA10380.17615373910918478No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA10310.1749658044523791No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9980.16936554107029517No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9970.16919583611932296No Hit
TCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCA9790.16614114700182264No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC9770.16580173709987817No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9310.15799530935515513No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9250.1569770796493217No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC8910.15120711131626555No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8530.14475832317932044No Hit
CAATAGGTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT8520.14458861822834818No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG8380.14221274891473684No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA8300.1408551093069589No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8280.14051569940501443No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8230.13966717465015324No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8080.13712160038556964No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8070.1369518954345974No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA8050.13661248553265293No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7990.1355942558268195No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA7950.13491543602293055No Hit
TAGATAAAGGCATTAAACCTTATTATCCTGAACACATAGTTAATCATTAC7910.1342366162190416No Hit
CTCCTGAACATTGTTCTCCTCACCATACAGCACTAAGGCAAGCCATTCTG7840.1330486815622359No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC7600.12897576273890216No Hit
CACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGT7590.12880605778792992No Hit
GTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATA7580.12863635283695765No Hit
ATATAAGACCTTGGGCAAGACTTGGCGGGCGTTCACGGTGGTTTCCATGC7570.1284666478859854No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG7550.12812723798404096No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7540.1279575330330687No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC7510.12744841818015198No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT7390.1254119587684851No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG7330.12439372906265167No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG7120.12082992509223464No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT6980.11845405577862328No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6900.11709641617084537No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC6790.11522966171015074No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6790.11522966171015074No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6760.11472054685723401No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6740.11438113695528952No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6720.11404172705334506No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6550.11115674288681698No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6510.11047792308292802No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6390.10844146367126113No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTGTAGGAAGTTTCCGAAAAC6280.1065747092105665No Hit
GGAAACGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTAACAT6270.10640500425959426No Hit
CTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT6200.1052170696027886No Hit
CCACAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6140.10419883989695516No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA6130.10402913494598291No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6050.102671495338205No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6010.10199267553431604No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6000.1018229705833438No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA5970.10131385573042709No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5920.10046533097556587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)