FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589258
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG50120.8505612142728652No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT31360.5321947262489436No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22290.3782723357171222No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG21810.3701264980704547No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG21480.3645262346883708No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG21410.36333830003156514No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG16900.286801367143085No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT16730.28391638297655697No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT16040.2722067413594724No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA15880.26949146214391656No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15060.2555756561641929No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC14780.2508239175369702No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA13980.23724752145919104No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG13910.23605958680238537No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA13840.23487165214557967No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAA12930.2194285016071059No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT12890.2187496818032169No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT12800.21722233724446677No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT12390.21026443425460495No Hit
TCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCA11600.196857743127798No Hit
ATTTTGTGGTCTGTAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACA11550.1960092183729368No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11470.1946515787651589No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG11450.1943121688632144No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGA11220.1904089549908529No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC11150.18922102033404722No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA11150.18922102033404722No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC11020.18701485597140813No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC10380.17615373910918478No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10310.1749658044523791No Hit
ATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA10180.17275964008973999No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10100.17140200048196205No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9680.164274392541128No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC9640.16359557273723904No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT9350.15867412915904408No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC9330.1583347192570996No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA8950.1518859311201545No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC8900.1510374063652933No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8420.1428915687186258No Hit
CTCCTGAACATTGTTCTCCTCACCATACAGCACTAAGGCAAGCCATTCTG8370.1420430439637646No Hit
ATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA8220.139497469699181No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8220.139497469699181No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8130.13797012514043086No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7910.1342366162190416No Hit
CAATAGGTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGT7890.1338972063170971No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA7870.1335577964151526No Hit
GTCTATTTGTAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATA7780.13203045185640247No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7710.13084251719959678No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC7640.1296545825427911No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGGAT7590.12880605778792992No Hit
AGCGAGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7500.12727871322917975No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC7450.12643018847431853No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7410.12575136867042958No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG7380.1252422538175129No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG7250.12303608945487376No Hit
CTTCTTCTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCT7240.12286638450390151No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG7220.12252697460195704No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC7140.12116933499417913No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTA7050.11964199043542896No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG7030.1193025805334845No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6980.11845405577862328No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6910.11726612112181761No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC6880.11675700626890088No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6750.11455084190626176No Hit
TAGATAAAGGCATTAAACCTTATTATCCTGAACACATAGTTAATCATTAC6620.11234467754362266No Hit
CTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT6610.11217497259265043No Hit
CACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGT6560.11132644783778924No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6520.11064762803390026No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT6460.10962939832806681No Hit
GCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAG6420.10895057852417786No Hit
CAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATT6410.10878087357320562No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6400.10861116862223338No Hit
GGAAACGTGAAACCACAATAGTTGTCTGATTTTTAGGCCCATATTAACAT6370.10810205376931667No Hit
GTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC6350.10776264386737218No Hit
GATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTA6310.10708382406348323No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG6280.1065747092105665No Hit
GTTTCTCTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATG6250.1060655943576498No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6200.1052170696027886No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC6200.1052170696027886No Hit
CTGTGTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG6160.10453824979889963No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6000.1018229705833438No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5990.10165326563237155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)