FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326843
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN49351.5098992482629274No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG22290.6819788093977842No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15590.4769874220956239No Hit
CTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGG15270.4671967886722372No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT15100.4619955146660629No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12070.3692904544383695No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAACCTCCTTTTTTCA9690.29647261835193045No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9140.2796449671554844No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8430.25792199924734505No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8220.2514968960632475No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7770.23772881781160987No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT7300.22334882497101055No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7250.22181903849860635No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG7170.2193713801427597No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT7020.21478202072554714No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA6860.20988670401385373No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT6810.20835691754144958No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6350.19428288199533109No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA6340.19397692470085023No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAACCTCCTTTTTTCATTAAC5800.1774552307988851No Hit
GGGTATACATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTA5680.17378374326511506No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG5530.1691943838479025No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5420.16582885360861332No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5300.16215736607484327No Hit
GGAACATATTGTACAAAAACTCAAACTATGTTTTCGAAAACTGCCTGTAA5300.16215736607484327No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5260.16093353689691994No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC5090.1557322628907457No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5010.15328460453489903No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC5000.15297864724041818No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC4980.15236673265145653No Hit
GTTTGGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4960.15175481806249486No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4780.1462475867618398No Hit
CTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGGTT4770.14594162946735895No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA4640.1419641846391081No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4430.1355390814550105No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGTACAATA4420.13523312416052968No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4320.1321735512157213No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGTACAAT4300.13156163662675965No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4260.1303378074488363No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4250.13003185015435545No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4150.1269722772095471No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4150.1269722772095471No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG4140.12666631991506624No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA4140.12666631991506624No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGT4020.12299483238129622No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA4000.12238291779233454No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC3960.12115908861441121No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3930.12024121673096869No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3850.11779355837512201No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3850.11779355837512201No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA3790.11595781460823698No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG3790.11595781460823698No Hit
ATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGTACAATATGTT3770.1153459000192753No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3740.1144280281358328No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3670.11228632707446695No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3620.11075654060206278No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT3610.11045058330758192No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT3600.1101446260131011No Hit
GAATGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3590.10983866871862025No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAAT3580.10953271142413942No Hit
CCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT3560.10892079683517775No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAATAAA3470.10616718118485022No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT3430.10494335200692687No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3390.10371952282900351No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA3390.10371952282900351No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGGAT3340.10218973635659934No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA3310.10127186447315684No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3300.10096590717867598No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)