FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326843
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG24810.759080047606955No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT15390.4708682762060072No Hit
CTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGG15340.46933848973360304No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13600.41610192049393746No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA12160.372044070088697No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11240.34389599899646006No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT10070.3080989955422022No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAACCTCCTTTTTTCA9850.30136793506362386No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8760.26801858996521266No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT8530.2609815721921534No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8190.250579024179805No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8160.2496611522963625No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA8020.24537775017363078No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAAGAGGGAGTTTG7730.23650498863368652No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCA7180.2196773374372405No Hit
CATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCAT7160.21906542284827885No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6570.20101394247390947No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6260.19152926634500356No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAACCTCCTTTTTTCATTAAC6220.1903054371670802No Hit
GGGTATACATTTAAACCCTAATAAAACCAAGCGTTGGGGCTACTCCCTTA5990.18326841939402097No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA5990.18326841939402097No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTACAGG5610.1716420422037492No Hit
GTTTGGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC5510.16858246925894083No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGTACAATA5430.16613481090309415No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5370.16429906713620912No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5330.1630752379582858No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5330.1630752379582858No Hit
GGAACATATTGTACAAAAACTCAAACTATGTTTTCGAAAACTGCCTGTAA5270.16123949419140077No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC5090.1557322628907457No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTC4990.15267268994593736No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC4940.15114290347353318No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCGAAAAC4560.1395165262832614No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA4500.13768078251637636No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4460.13645695333845304No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4400.134621209571568No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4360.13339738039364465No Hit
CTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGGTT4350.13309142309916383No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4310.13186759392124048No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC4120.1260544053261046No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG4120.1260544053261046No Hit
ATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGTACAATATGTT4110.12574844803162374No Hit
GGTCTATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGTACAAT4110.12574844803162374No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4060.12421866155921957No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4000.12238291779233454No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA3990.12207696049785371No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAAT3940.12054717402544954No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAA3920.11993525943648786No Hit
CAATAGGTCTATTTACAGGCAGTTTTCGAAAACATAGTTTGAGTTTTTGT3910.11962930214200701No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3880.11871143025856451No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA3800.11626377190271783No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG3680.11259228436894778No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3680.11259228436894778No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3640.11136845519102442No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT3610.11045058330758192No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA3610.11045058330758192No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT3610.11045058330758192No Hit
CATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT3610.11045058330758192No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3590.10983866871862025No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3580.10953271142413942No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAA3530.10800292495173525No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT3470.10616718118485022No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA3470.10616718118485022No Hit
ATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGGAT3470.10616718118485022No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGGTTAAAATTAATTA3460.1058612238903694No Hit
CTATTACCAATTTTCTTTTGTCTCTGGGTATACATTTAAACCCTAATAAA3420.10463739471244604No Hit
ATTTACAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT3330.10188377906211851No Hit
GAAAAAAGGAGGTTAAAATTAATTATGCCTGCTAGGTTCTATCCTAACCT3320.10157782176763766No Hit
CCCTAGCAGCCATGGAAATGAGGTGTATTTCCGAGAGAGGACAACAGAGT3310.10127186447315684No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3310.10127186447315684No Hit
GAATGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3280.10035399258971434No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG3270.10004803529523348No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAAC3270.10004803529523348No Hit
TAATAAAACCAAGCGTTGGGGCTACTCCCTTAACTTCATGGGATATGTAA3270.10004803529523348No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)