FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences500344
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN34730.694122443758694No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13260.26501766784452296No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA12670.2532257806629039No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT11710.23403898118094751No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11200.22384599395615817No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCTCTGACATACT10760.21505204419359478No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10380.20745726939865372No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9770.19526565722782727No Hit
CCCTTAACTTCATGGGATATGTAATTGGATGTTGGGGTACTTTACCGCAA9150.18287418256239707No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9150.18287418256239707No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9000.17987624514334138No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA8810.17607885774587084No Hit
ATGTAATTGGATGTTGGGGTACTTTACCGCAAGAACATATTGTACTAAAA8740.17467982028364484No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8470.16928353292934462No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG8370.16728490798330747No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTTCCAATTTTCTT8210.16408710806964807No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8090.16168875813440353No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAATGTTAATATGGGC7840.1566921957693107No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7810.15609260828549956No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7430.14849783349055848No Hit
CTCCAAAGGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7030.14050333370641No Hit
GTTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTAGTACAATATGTT6710.13410773387909117No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6540.13071007147082808No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGACGTTCACGGTGGTCTCCATGC6500.12991062149241323No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAGGGCATTAAACCATAT6500.12991062149241323No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATACTGTGGTTTCACATTTCC6480.1295108965032058No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6460.12911117151399837No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6290.12571350910573525No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6270.12531378411652783No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTTCCAATTTTCTTTTGTC6200.12391474665430183No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6140.12271557168667957No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6100.12191612170826471No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCAGGGTCCCGTG6070.12131653422445357No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCATAT5860.11711942183777561No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5730.11452120940792734No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA5730.11452120940792734No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5690.11372175942951249No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5640.11272244695649393No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGAAATAGAGGTAAAAA5500.10992437203204196No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5450.1089250595590234No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCCTTTCCTCAC5380.10752602209679739No Hit
CTCTAAGGCCTCCCGATACAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5370.10732615960219369No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC5320.10632684712917513No Hit
CTGACATACTTTCCAATCAATAGGTCTGTTTACAGGCAGTTTTCTAAAAC5280.10552739715076027No Hit
ATGCTGGATCGTCCAAATTACTTCCCACCCAGGTGGCCAGGTTCATCAAC5200.10392849719393059No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT5190.10372863469932686No Hit
CAATAGGTCTGTTTACAGGCAGTTTTCTAAAACATTGCTTGATTTTTAGT5140.1027293222263083No Hit
CCTTTGGAGTGTGGATTCGCACTCCTACTGCTTACAGACCACCAAATGCC5080.10153014725868602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)