FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences541439
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTACAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT28650.5291454808390235No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCCGG27190.5021803010126719No Hit
ATTTAAAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT23500.43402857939675565No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT22170.4094644087330244No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21270.3928420376071913No Hit
ATGTAGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATG16900.3121311911406456No Hit
ACAATATGTAGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGA16890.3119464981281363No Hit
GGATATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAATTAACG16370.3023424614776549No Hit
CTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG15320.2829496951641829No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15070.2783323698514514No Hit
TTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAATTAACGAAAAA14530.2683589471759515No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT14360.2652191659632941No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13680.25266004111266455No Hit
GTTCTATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC13100.24194784638712766No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACAGACT12770.23585297697432211No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT11860.21904591283597968No Hit
CCTTTAAATAGACCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGGCT11460.21165819233560937No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG11270.20814902509793345No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11090.20482455087276683No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG10630.19632867229734097No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10410.1922654260221373No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT10310.19041849589704474No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9560.1765665199588504No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9360.17287265970866522No Hit
TATTAATAGATGTCAACAATATGTAGGCCCTCTTACAATTAACGAAAAAA9310.17194919464611894No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT9070.1675165623458968No Hit
GAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA8720.16105230690807273No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC8700.16068292088305422No Hit
ATATGTAGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATTGATTA8590.1586512977454524No Hit
ATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTGCCAGTC8550.15791252569541536No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCC8450.1560655955703228No Hit
ATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8130.15015541917002656No Hit
CTATTAATAATATCCTCTTTCAAATGAATATGAGGAAAAGAGGGAGTTTG8130.15015541917002656No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7750.14313708469467476No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAT7710.14239831264463773No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7660.14147484758209145No Hit
CTTACAATTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGGTT7380.13630344323183222No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATTTGGCCAC7280.13445651310673964No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7240.13371774105670262No Hit
GATTAACTATGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6920.12780756465640636No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA6600.12189738825611011No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6510.12023515114352677No Hit
CATGTATACAAGCTAAGCGGGCTTTCACTTTCTCGCCAACTTACAAGGCC6500.12005045813101754No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6460.11931168608098051No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG6440.11894230005596199No Hit
ATTATTAATAGATGTCAACAATATGTAGGCCCTCTTACAATTAACGAAAA6400.11820352800592496No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC6350.11728006294337867No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6290.11617190486832311No Hit
GGTCTATTTAAAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT6280.11598721185581386No Hit
GATGTCAACAATATGTAGGCCCTCTTACAATTAACGAAAAAAGGAGATTA6260.11561782583079534No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6110.11284743064315647No Hit
ACTATGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA6110.11284743064315647No Hit
ATTAATAGATGTCAACAATATGTAGGCCCTCTTACAATTAACGAAAAAAG6100.11266273763064721No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6080.11229335160562871No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT6080.11229335160562871No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTTCGAGAGAGGACAACAGAGT6050.11173927256810093No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5980.11044642148053613No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5960.11007703545551761No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCCGGATAATAAGGTTTAATGCC5960.11007703545551761No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTCTGTCAACGAATTGTGGGGCT5910.10915357039297131No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT5900.10896887738046207No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5870.10841479834293428No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5800.1071219472553695No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5710.10545971014278618No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5700.10527501713027691No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5700.10527501713027691No Hit
TGATTAACTATGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGG5690.10509032411776766No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC5680.10490563110525838No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC5630.1039821660427121No Hit
CAACAATATGTAGGCCCTCTTACAATTAACGAAAAAAGGAGATTAAAATT5590.10324339399267507No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5530.10213523591761951No Hit
ATGATTAACTATGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGG5490.1013964638675825No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5480.10121177085507325No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAAAATTTAATCTA5480.10121177085507325No Hit
ATGTAAGACCTTGGGCAACACCTGTCGGGCGTTCACGGTGGTCTCCATGC5470.10102707784256398No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)