FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531592
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN39430.741734262366627No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT21990.41366311005432743No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA18580.3495161702960165No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT17660.33220966455477136No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA16270.306061791750064No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT15040.2829237460307905No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA13870.26091438546855483No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA13480.2535779319478096No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG12780.24040993844903613No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12090.22743005914310221No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11600.2182124636939608No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT11430.21501452241568722No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT11410.21463829403000795No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGATTGGCAAACCCCTTCTTTTC10720.2016584147240741No Hit
CCTATTAGCAATATCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT10240.19262893346777227No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG10080.18961910638233834No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG10040.18886664961097985No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT9160.17231260064109316No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9100.17118391548405543No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8960.16855031678430074No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA8530.16046140649219703No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG8530.16046140649219703No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8500.15989706391367817No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8440.15876837875664043No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8380.1576396935996027No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA8300.15613478005688575No Hit
CTTTAATCCTGATTGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG8080.15199626781441405No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT8050.1514319252358952No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT7950.149550783307499No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA7860.1478577555719424No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7750.14578849945070654No Hit
ATAGACTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAA7720.14522415687218768No Hit
CATGAAGATATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT7700.14484792848650846No Hit
CTGACATACTTTCCAATCAATAGGTCGATTGACAGGAAGTTTTCTAAAAC7600.14296678655811224No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC7550.14202621559391412No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA7430.13976884527983868No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA7080.13318484853045193No Hit
GGACCTTGACCATTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCAT6970.13111559240921608No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA6870.12923445048081986No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT6750.12697708016674442No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC6370.11982874083883881No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC6310.11870005568180109No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCGAAACAGGCTTTTACTTT6110.11493777182500867No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT6070.11418531505365016No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5870.11042303119685774No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5730.10778943249710304No Hit
CCTTTAGACAAAGGGATTAAACCTTATTATCCTGATCATGTAGTTAATCA5700.10722508991858418No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5690.10703697572574455No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5550.10440337702598985No Hit
GTACAATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCC5520.103839034447471No Hit
ATGTAAGACCTTGGGCAGGACCTGATGGGCGTTCACGGTGGTCTCCATGC5520.103839034447471No Hit
GTTAATGACTCTAGCTACCTGGGTGGGTAATAATTTGGAAGATCCAGCAT5500.10346280606179174No Hit
CTACATGATCAGGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATAT5430.10214600671191441No Hit
ACTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGATGGAAATTGC5430.10214600671191441No Hit
GTGTAGTTTCCCTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAA5430.10214600671191441No Hit
GGGTAACCACATTGTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAAT5410.10176977832623516No Hit
GGATAAAACCTAGCAGGCATAATTAATTTCAGTCTTCTTTTTTCATTTAC5400.10158166413339555No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5390.10139354994055591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)