FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488662

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488662
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479468
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACCCTGATAATCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG22550.4703129301642654No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC20600.4296428541633644No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTCT19730.4114977433321932No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGG17060.3558110238848057No Hit
ATATGATCCTGTGGCAATGAACCCCAACTTCCAATTACATATCCCATGAA16620.34663418622306386No Hit
ACTGCTGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG16600.34621705723843926No Hit
ATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAACG16340.3407943804383191No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTATT14370.2997071754527935No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT12930.2696738885598205No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG12410.2588285349595802No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA12290.25632576105183247No Hit
CATACAAAGGCATTAAGGCAGGGTACCCACATTGTGTGAATGGAGCGGCA11940.2490260038209015No Hit
CCTATTAATAATGTCCTCATGTAAATGAATTTTAGGAAAAGAAGGAGTTT11870.2475660523747153No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10730.22378970025111164No Hit
GGATCATATTGTACAAAAAATCAAACAATGTTTTAGAAAATTGCCTGTCA10450.21794989446636692No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTTCTTTTC10210.2129443466508714No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCCACAACAACC10180.21231865317393445No Hit
TGGTTGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTGTGTCTTGGCCA10100.21065013723543594No Hit
CTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACT9190.1916707684350155No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG9160.19104507495807854No Hit
AGTCCACCACGAGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACA8770.18291105975789834No Hit
CTCCGAAAGAGACCAAATATTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8690.18124254381939983No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA8690.18124254381939983No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTCTGGAAGTAATGATTAACTACT8360.1743599155730935No Hit
TTATTATCCTGATCAAGTAGTTAATCATTACTTCCAGACCCGACATTATT8290.1728999641269073No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT8280.172691399634595No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGCTATTCCCTGAATTTCAT8260.1722742706499704No Hit
CTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATATTC7950.16580877138828867No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACGGGG7860.1639316909574779No Hit
TACCTTGATAATCCAGAAGAACCAATAAGAAGATGAGGCATAGCAGCAGG7730.16122035255741782No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAATTTTCTAGGGGGAGCACCCGTG7550.15746619169579618No Hit
TTATTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAA7490.15621480474192231No Hit
ATATAGAGCTGAGGCGGTATCTAGTAGATCCCGTACTGAAGGAAAGAAGT7110.1482893540340544No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGTAGATCCCGTA6910.14411806418780815No Hit
CTCTCAACCCTGTTCCAAATATTGCCTCTCACATCTCGTCAATCTCCTCG6870.1432838062185589No Hit
CTTTAATCCTGAATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATG6840.14265811274162196No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6790.1416152902800604No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6650.13869538738768802No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6600.13765256492612646No Hit
CTCCATATTCGGCGCAGGGTCCCCAGTCCTCGAGGAGATTGACGAGATGT6530.13619261347994027No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC6500.13556692000300333No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6500.13556692000300333No Hit
GGGTAAAGGTTCACATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6400.1334812750798802No Hit
GTATGTAAATAATGTCGGGTCTGGAAGTAATGATTAACTACTTGATCAGG6390.13327271058756787No Hit
GGTCCTAGGAATCCTGATGTGATGTTCTCCATATTCGGCGCAGGGTCCCC6380.13306414609525558No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6320.13181275914138169No Hit
GTCCCGTACTGGTTGTTGTGGATCCTGGAATTAGAGGACAAACGGGCAAC6280.13097850117213244No Hit
GTTCACATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6260.13056137218750782No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA6130.12785003378744778No Hit
GGTCTATAGGGTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATA6070.12659864683357389No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCA6050.12618151784894927No Hit
GCTTTATTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACTCCTT5910.12326161495657688No Hit
CTCTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5810.12117597003345375No Hit
ATACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5810.12117597003345375No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5730.11950745409495525No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5540.1155447287410213No Hit
ATGTAAGACCTTGGGCAAGATCTGATGGGCGTTCACGGTGGTCTCCATGC5470.11408477729483511No Hit
GACCAGGCCGTTGCCGGGCAACGGGGTAAAGGTTCACATACTGTTTACTT5450.11366764831021048No Hit
GCCCCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAAGAGTCCTCTTATG5440.11345908381789817No Hit
CTTGTACATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT5360.11179056787939967No Hit
TGTCAGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCAT5260.10970492295627654No Hit
GATCTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGG5250.10949635846396423No Hit
GTCTAGAGGCAAATATTTAGTGCTATTAGGATAAAATCTAGCAGGCATAA5210.10866210049471497No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACGGGGCTTTATTCTTCTACTG5180.10803640701777803No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5050.10532506861771797No Hit
CATATAAAGGCATTAAGGCAGGGTACCCACATTGTGTGAATGGAGCGGCA5000.1042822461561564No Hit
ACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAATTA4940.10303085920228253No Hit
GCCAACAGTTCCTCCTCCTGCCTCCACCAATCGGCAGTCAGGAAGGCAGC4920.1026137302176579No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4900.10219660123303327No Hit
ACTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC4840.1009452142791594No Hit
ATTTAGTGCTATTAGGATAAAATCTAGCAGGCATAATTAATTTCAGTCTT4810.10031952080222245No Hit
GAATACAGGTGCAATTTCCATCCGTAGGTTTTGTACAGCAACATGAGGGA4800.10011095630991014No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)