FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480372
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57611.1992788921918847No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT16290.3391121880542579No Hit
GCTAATAGGTGTGAACAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG11820.2460593040393695No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTTGTGCTGTT10210.21254361203400696No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT9810.20421673203267465No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9600.19984512003197522No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8990.18714662802994345No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8900.18527308002964368No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8690.18090146802894422No Hit
CCATTTACACAATGTGGTTATCCTGCATTAATGCCTTTATATGCATGCAT8560.17819523202851123No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8370.1742399640278784No Hit
CTCAAGGACAGTATCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA8110.16882749202701242No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT7830.1629986760260798No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA7200.14988384002398142No Hit
TTATTATCCTGATCAGGTAGTTAATCATTACTTTCAAACCCGACATTATT7030.14634491602341518No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTATCTCTTCCAAAAGTAAGG7000.14572040002331527No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC6880.1432223360229156No Hit
CATGAAGACATTGCTAATAGGTGTGAACAATTTGTAGGCCCTCTCACTGT6540.1361444880217831No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6250.1301075000208172No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACC6130.1276094360204175No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT6120.1274012640203842No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGATTGGCAAACCCCTTCTTTTC6110.1271930920203509No Hit
GGGTAAAGGTTCACATATTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA6010.12511137202001782No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACCTGATCAGG5760.11990707201918513No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGATCACCCGTG5590.11636814801861889No Hit
ATGTGATCTTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA5490.11428642801828581No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5310.1105393320176863No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGGAAAACCCC5260.10949847201751975No Hit
GTGCTGGGACGACTGCATATCCACATGCAAAAGAATGAAATTGGATCCCT5220.10866578401738651No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5150.10720858001715339No Hit
GTCTAGAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA5060.1053350320168536No Hit
ACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5030.10471051601675369No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT5000.10408600001665376No Hit
ATGTAAGACCTTGGGCAGGATCTGATGGGCGTTCACGGTGGTCTCCATGC4990.10387782801662045No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT4950.10304514001648724No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC4920.1024206240163873No Hit
CATATAAAGGCATTAATGCAGGATAACCACATTGTGTAAATGGAGCGGCA4890.10179610801628737No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4890.10179610801628737No Hit
CTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTT4850.10096342001615416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)