FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488665

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488665
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480372
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN57291.1926173881908186No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT15440.32141756805142685No Hit
GCTAATAGGTGTGAACAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG11830.2462674760394028No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10360.2156661920345066No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTTGTGCTGTT9790.20380038803260808No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT9300.193599960030976No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9240.19235092803077614No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA8970.18673028402987685No Hit
CCATTTACACAATGTGGTTATCCTGCATTAATGCCTTTATATGCATGCAT8830.18381587602941055No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8230.1713255560274121No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTATCTCTTCCAAAAGTAAGG7890.16424770802627964No Hit
CTCAAGGACAGTATCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA7780.16195781602591325No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA7380.15363093602458094No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTGAATTTCAT7190.1496756680239481No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7060.1469694320235151No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC6740.14030792802244926No Hit
TTATTATCCTGATCAGGTAGTTAATCATTACTTTCAAACCCGACATTATT6640.1382262080221162No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT6580.13697717602191636No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTCGGGGGATCACCCGTG6550.13635266002181642No Hit
GGGCTGTATCGTCCCCTTCTTCGTCTGCCGTACCGTCCGACCACGGGGCG6310.13135653202101705No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACCTGATCAGG6240.12989932802078388No Hit
CCTCCACCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6180.12865029602058406No Hit
GGGTAAAGGTTCACATATTGTTGACTTAGAAAGGCCTTGTAAGTTGGCGA6050.12594406002015107No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5940.12365416801978467No Hit
TTCTTCTACTGTCCCTATCTTTAATCCTGATTGGCAAACCCCTTCTTTTC5900.12282148001965143No Hit
CATGAAGACATTGCTAATAGGTGTGAACAATTTGTAGGCCCTCTCACTGT5830.12136427601941828No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACC5790.12053158801928504No Hit
GTCTAGAGGCAAATATTTTGTGCTGTTAGGATAAAACCTAGCAGGCATAA5490.11428642801828581No Hit
ATGTGATCTTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA5480.11407825601825253No Hit
CTCTAAGGCTTCCCGATACAGAGCTGAGGCTGTATCTAGTAGATCCCGGA5430.11303739601808598No Hit
CTCTTGTACATGTCCCACTTTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC5070.10554320401688691No Hit
GTGCTGGGACGACTGCATATCCACATGCAAAAGAATGAAATTGGATCCCT5070.10554320401688691No Hit
GCCTTAGAGTCTCCTGAGCATTGCTCACCTCACCATACAGCACTCAGGCA5030.10471051601675369No Hit
TCCCGATACAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAA4900.10200428001632068No Hit
GTATTAACATAATTGACTACTAGATCCCTGGATGCTGGATCTTCCAAATT4900.10200428001632068No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT4890.10179610801628737No Hit
ACTAGATACAGCCTCAGCTCTGTATCGGGAAGCCTTAGAGTCTCCTGAGC4810.10013073201602092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)