FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273968
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC21150.7719879693978858No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16460.6008000934415698No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13380.4883782047538398No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10810.3945716288033639No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8400.30660515096653623No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC7300.26645447643520415No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7140.2606143783215558No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7060.2576943292647316No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT6840.2496641943584652No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6290.2295888570927992No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTACG6170.2252087835075629No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAGTACTCTATGA5790.21133855048764819No Hit
TCCTAATACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT5770.21060853822344214No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCTTTAGAGTCTCCTGA5720.20878350756292707No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC5270.1923582316182912No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5260.19199322548618816No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5250.19162821935408517No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5210.19016819482567307No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5200.18980318869357005No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATGTCGTACTGAAGGAAAAAAGT5170.188708170297261No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACATTTCAT5150.18797815803305495No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5150.18797815803305495No Hit
ATCTAGAAGATGTCGTACTGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA5030.18359808444781872No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5030.18359808444781872No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5000.18250306605150968No Hit
ATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC4880.17812299246627342No Hit
ACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4870.17775798633417042No Hit
CTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC4800.17520294340944928No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC4700.1715528820884191No Hit
GAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGAACAGTAGAAGAA4670.17045786369211002No Hit
ATTTATGCCTACAGCCTCCTAATACAAACAACTTTAATCTAATCTCCTCC4610.1682678268994919No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4600.16790282076738888No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4500.1642527594463587No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4470.16315774105004963No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4450.1624277287858436No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4420.16133271038953453No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4350.15877766746481342No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG4350.15877766746481342No Hit
GCTCTATATCGGGAAGCTTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4320.15768264906850435No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4280.15622262454009228No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT4230.1543975938795772No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4010.14636745897331074No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3960.14454242831279565No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3960.14454242831279565No Hit
CTCTAAAGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATGTCGTA3930.1434474099164866No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3900.14235239152017753No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3880.1416223792559715No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAACAACTTTAATCTAATCT3790.13833732406704433No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3790.13833732406704433No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGC3750.13687729953863226No Hit
GTATGCATGTATTCAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACA3720.13578228114232319No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3710.1354172750102202No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3680.13432225661391112No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG3670.1339572504818081No Hit
GTCATAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3660.13359224434970507No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3650.13322723821760207No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA3650.13322723821760207No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3640.13286223208549905No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3630.132497225953396No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3530.1288471646323658No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT3520.1284821585002628No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG3500.12775214623605677No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA3480.12702213397185072No Hit
CCCTCCTTTCCTAATATACATTTACACCAAGACATTATCAAAAGATGTGA3410.12446709104712958No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA3360.1226420603866145No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC3360.1226420603866145No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA3320.12118203585820243No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3310.1208170297260994No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT3300.12045202359399637No Hit
GAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGT3290.12008701746189336No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA3260.11899199906558429No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC3250.11862699293348129No Hit
AATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTT3250.11862699293348129No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG3240.11826198680137825No Hit
GTCCTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3240.11826198680137825No Hit
GCATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG3210.11716696840506921No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3190.11643695614086318No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3170.11570694387665711No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3150.11497693161245108No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3150.11497693161245108No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3140.11461192548034807No Hit
CCTAATACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC3110.11351690708403901No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCAAAGC3110.11351690708403901No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC3100.113151900951936No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3090.11278689481983298No Hit
CATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATT3080.11242188868772997No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3030.11059685802721486No Hit
GCTTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA3030.11059685802721486No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG3010.10986684576300883No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT3010.10986684576300883No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCAAAGCAGGCTTTCACTTT3000.10950183963090579No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG3000.10950183963090579No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2990.1091368334988028No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG2980.10877182736669976No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTTGCTAGAGTCATTAGT2960.10804181510249372No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA2930.10694679670618465No Hit
GTATGATGTGATCTTGTGGCAATGAACCATAACATCCAATGACATAACCC2930.10694679670618465No Hit
CATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGC2920.10658179057408165No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA2910.10621678444197862No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG2890.10548677217777258No Hit
GTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGG2890.10548677217777258No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2890.10548677217777258No Hit
TAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTCCAAATTACCA2860.10439175378146354No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG2790.1018367108567424No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG2790.1018367108567424No Hit
GTAAAGAGTAACCCCATCTTTTCGTTTTGTTAGGGTTTAAATGTATACCC2760.10074169246043334No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC2760.10074169246043334No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA2750.10037668632833033No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA2740.10001168019622729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)