FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273968
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAACAAC19480.7110319453366817No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA19200.7008117736377971No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13330.4865531740933248No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9840.359166033989371No Hit
GTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8030.29309992407872454No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7650.2792296910588098No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA7250.26462944577468905No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTTTTTCCTTCAGTACG6710.24491911464112598No Hit
CCTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTAT6250.22812883256438707No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTC6230.22739882030018105No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAGTACTCTATGA5600.2044034339776908No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAAGAAT5600.2044034339776908No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCTTTAGAGTCTCCTGA5590.20403842784558782No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5590.20403842784558782No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5450.19892834199614554No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC5110.18651813350464289No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5070.18505810897623082No Hit
GGATAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAAT4940.18031302925889156No Hit
CATTTAAACCCTAACAAAACGAAAAGATGGGGTTACTCTTTACATTTCAT4870.17775798633417042No Hit
ACCCTTCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCG4840.17666296793786135No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC4830.17629796180575835No Hit
TCCTAATACAAACAACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT4800.17520294340944928No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC4770.1741079250131402No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4760.1737429188810372No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATGTCGTACTGAAGGAAAAAAGT4700.1715528820884191No Hit
GAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGAACAGTAGAAGAA4660.170092857560007No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT4600.16790282076738888No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA4570.16680780237107984No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4560.16644279623897681No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4540.16571278397477077No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG4460.16279273491794663No Hit
ATTTATGCCTACAGCCTCCTAATACAAACAACTTTAATCTAATCTCCTCC4450.1624277287858436No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4350.15877766746481342No Hit
ATCTAGAAGATGTCGTACTGAAGGAAAAAAGTCAGAAGGCAAAAACGAGA4350.15877766746481342No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4270.15585761840798926No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4240.1547626000116802No Hit
GCTCTATATCGGGAAGCTTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4110.15001752029434096No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG4100.14965251416223793No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4040.14746247736961982No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG3970.14490743444489868No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3970.14490743444489868No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG3930.1434474099164866No Hit
GTATGCATGTATTCAATCAAAGCAGGCTTTCACTTTCTCGCCAACTTACA3890.14198738538807454No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC3890.14198738538807454No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT3830.1397973485954564No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT3820.1394323424633534No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT3790.13833732406704433No Hit
GTCATAGAGTACTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC3780.1379723179349413No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3760.13724230567073528No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3750.13687729953863226No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3730.1361472872744262No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3670.1339572504818081No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA3650.13322723821760207No Hit
CTCTTATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTC3650.13322723821760207No Hit
CTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGGAGACC3630.132497225953396No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCCCACCAATTCTTGCCC3610.13176721368918998No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3540.12921217076446886No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT3530.1288471646323658No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG3520.1284821585002628No Hit
CTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGC3500.12775214623605677No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3500.12775214623605677No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC3470.12665712783974772No Hit
CTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA3460.12629212170764467No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3440.12556210944343865No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT3430.12519710331133563No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3400.12410208491502657No Hit
ATGCAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC3370.12300706651871751No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG3370.12300706651871751No Hit
CCCTCCTTTCCTAATATACATTTACACCAAGACATTATCAAAAGATGTGA3340.12191204812240847No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3340.12191204812240847No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA3310.1208170297260994No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG3270.11935700519768733No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCAAAGC3180.11607195000876015No Hit
GACCAATTTATGCCTACAGCCTCCTAATACAAACAACTTTAATCTAATCT3170.11570694387665711No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACGGGGCGCACCT3160.11534193774455412No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC3130.11424691934824505No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT3120.11388191321614204No Hit
GTCTTGCATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT3100.113151900951936No Hit
GCTTTAATGCCCTTGTATGCATGTATTCAATCAAAGCAGGCTTTCACTTT3090.11278689481983298No Hit
TAACCACATTGTGTAAAAGGAGCAGCAAAACCCAAAAGACCCACAATACG3060.1116918764235239No Hit
CCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGAAGTATGCCTCA3060.1116918764235239No Hit
ACTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG3060.1116918764235239No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC3060.1116918764235239No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA3050.1113268702914209No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3050.1113268702914209No Hit
CTTCAAAGACTGTTTGTTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA3040.11096186415931787No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGAGC3040.11096186415931787No Hit
CTCTAAAGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATGTCGTA3020.11023185189511184No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2990.1091368334988028No Hit
ATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCA2980.10877182736669976No Hit
ATATTGGATCTTCCAAATTACCACCCACCCAGGTTGCTAGAGTCATTAGT2950.1076768089703907No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT2940.10731180283828769No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG2940.10731180283828769No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA2940.10731180283828769No Hit
GCATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGG2920.10658179057408165No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA2920.10658179057408165No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG2910.10621678444197862No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGATCCTTAT2880.10512176604566957No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC2840.10366174151725747No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2790.1018367108567424No Hit
CTCTTTACATTTCATGGGTTATGTCATTGGATGTTATGGTTCATTGCCAC2780.10147170472463937No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA2780.10147170472463937No Hit
ATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCAGGTCTGGAGCA2770.10110669859253636No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC2770.10110669859253636No Hit
ACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT2760.10074169246043334No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTT2740.10001168019622729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)