FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423185
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18840.44519536372981083No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16700.3946264636033886No Hit
ATATAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC12650.2989236385977764No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC10160.24008412396469628No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9340.22070725569195504No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT8380.19802214161655068No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACTCCCTCTTTTCCCAAT7960.18809740420856128No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7730.18266242896132898No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA7690.1817172158748538No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7640.1805356995167598No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7540.17817266680057187No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC7540.17817266680057187No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7180.16966574902229523No Hit
CAATAACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG7130.16848423266420123No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTCCTGACTTCTTTCCTTCAGTACG6950.16423077377506293No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT6910.16328556068858774No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT6830.16139513451563736No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6760.1597410116143058No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6720.1587957985278306No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6680.15785058544135544No Hit
CTTATATAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCA6650.15714167562649906No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6500.15359712655221713No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6490.1533608232805983No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6210.14674433167527207No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC6190.14627172513203446No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT6160.14556281531717807No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6150.14532651204555927No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG6110.1443812989590841No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5950.14060044661318336No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGC5920.13989153679832697No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA5860.1384737171686142No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5760.13611068445242624No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5720.13516547136595108No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5690.13445656155109467No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5610.1325661353781443No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5560.13138461902005033No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5550.13114831574843153No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5550.13114831574843153No Hit
CACCAGGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5500.12996679939033756No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5460.12902158630386237No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5430.128312676489006No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC5430.128312676489006No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5430.128312676489006No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5360.12665855358767442No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5310.12547703722958045No Hit
GGATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA5260.12429552087148646No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5230.12358661105663009No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5220.12335030778501128No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5190.1226413979701549No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5180.1224050946985361No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA5170.1221687914269173No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5170.1221687914269173No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG5140.12145988161206091No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5070.11980575871072935No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC5040.11909684889587295No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5020.11862424235263537No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4980.11767902926616018No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4980.11767902926616018No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4980.11767902926616018No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT4930.11649751290806622No Hit
AGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA4890.11555229982159103No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4860.11484339000673466No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4850.11460708673511584No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4840.11437078346349706No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGGAAAATTCCTATGGGAG4730.1117714474756903No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4680.11058993111759632No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTAAAGCCTCCTAATAATTAATCT4550.10751798858655197No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT4540.10728168531493319No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT4500.106336472228458No Hit
GCTTTAATGCCTTTGTATGCATGTATTCAATCTAAGCAGGCTTTCACTTT4500.106336472228458No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4490.1061001689568392No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACTCCCTCTTTTC4410.10420974278388885No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4390.10373713624065126No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4340.10255561988255726No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4270.1009014969812257No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4270.1009014969812257No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT4240.1001925871663693No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)