FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences423185
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19150.45252076514999356No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18130.42841783144487633No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC11080.2618240249536255No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACTCCCTCTTTTCCCAAT9390.221888772050049No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9360.22117986223519265No Hit
ATATAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC8410.1987310514314071No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8180.19329607618417477No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8100.1914056500112244No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCATACAGAAA8040.18998783038151162No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8020.18951522383827404No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7680.18148091260323498No Hit
CAATAACATAGCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTG7350.17368290463981473No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACATAAACC7220.1706109621087704No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7180.16966574902229523No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6970.1647033803183005No Hit
CTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGGA6870.16234034760211255No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCT6820.16115883124401856No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT6740.1592684050710682No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6710.15855949525621182No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6660.15737797889811786No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTCCTGACTTCTTTCCTTCAGTACG6510.1538334298238359No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6290.1486347578482224No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6210.14674433167527207No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6130.1448539055023217No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG6110.1443812989590841No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT6080.1436723891442277No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5990.14154565969965854No Hit
CTATAAGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5970.14107305315642096No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA5880.13894632371185178No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATAACATAGC5850.1382374138969954No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5850.1382374138969954No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5780.13658329099566385No Hit
ATCTTAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGG5610.1325661353781443No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC5610.1325661353781443No Hit
TAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5510.13020310266195634No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5480.12949419284709998No Hit
GGATATTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA5430.128312676489006No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5370.1268948568592932No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5360.12665855358767442No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5290.12500443068634287No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5290.12500443068634287No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCAAGC5280.12476812741472405No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5220.12335030778501128No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5210.1231140045133925No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5130.12122357834044213No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA5100.12051466852558573No Hit
ACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGAGCT5100.12051466852558573No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5030.11886054562425417No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5000.11815163580939778No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT4970.11744272599454139No Hit
CACCAGGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4930.11649751290806622No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4930.11649751290806622No Hit
GCTTTGGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG4900.11578860309320983No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4870.11507969327835343No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4850.11460708673511584No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT4810.11366187364864067No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4790.11318926710540307No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4690.11082623438921511No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4650.10988102130273993No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4650.10988102130273993No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4650.10988102130273993No Hit
GTCTTGGTGTAAATGTATATTGGGAAAAGAGGGAGTTTTCCAATGAGGGT4640.10964471803112115No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4590.10846320167302716No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT4540.10728168531493319No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA4530.10704538204331437No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4470.1056275624136016No Hit
GGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAA4440.10491865259874524No Hit
GCTTAGATTGAATACATGCATACAAAGGCATTAAAGCAGGATAACCACAT4430.10468234932712644No Hit
AGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAGACTA4420.10444604605550764No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4420.10444604605550764No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4410.10420974278388885No Hit
CTTATATAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCA4390.10373713624065126No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4290.1013741035244633No Hit
ATGTTATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACAGAAA4260.1006651937096069No Hit
GTTATGTTAACACTCATATGGGCCTAAAGTTTAGACAACTATTGTGGTTT4260.1006651937096069No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT4250.10042889043798812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)