FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540716
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA42690.7895087254677132No Hit
ATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAG26700.49378971585823245No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAGTTCGTAGGCCCACT23960.44311616449300556No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT22940.4242522877074102No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC21810.40335407126846623No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18400.34028954201466205No Hit
GCCACAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA16620.3073702276241132No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT16080.29738346932585685No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC15680.2899858705864076No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT15010.2775948926978303No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT14350.26538885477773916No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT13770.25466233660553783No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG12690.23468882000902508No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT12050.2228526620259064No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT11970.22137314227801655No Hit
ATGTCATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGAAT11770.217674342908292No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC11580.21416048350705363No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11520.21305084369613622No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT11430.21138638397976017No Hit
GTGTTGATTCTCTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAA11390.21064662410581525No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC11190.20694782473609066No Hit
CCCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCATC10680.19751588634329295No Hit
GTTCGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA10630.1965911865008618No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC10630.1965911865008618No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG10030.18549478839168806No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT9880.18272068886439463No Hit
CACCAAGATATTATCAAAAAATGTGAACAGTTCGTAGGCCCACTCACAGT9530.17624778996737658No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT9420.17421345031402807No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA9310.17217911066067956No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG9180.16977489107035856No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT9170.16958995110187233No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT9010.16663091160609267No Hit
CCATATACTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACCA8790.16256223229939562No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG8740.16163753245696447No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA8740.16163753245696447No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8670.16034295267756085No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8510.1573839131817812No Hit
TCGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG8410.15553451349691888No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT8380.15497969359146022No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8220.15202065409568055No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC8160.15091101428476317No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG8020.14832185472595596No Hit
CCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCATCT7840.1449929352932038No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7500.13870497636467202No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7490.1385200363961858No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7410.13704051664829597No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7280.13463629705797497No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7260.1342664171210025No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC7210.1333417172785714No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC7060.13056761775127795No Hit
ATCTCTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGT7000.12945797794036057No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6980.1290880980033881No Hit
ATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG6910.1277935182239845No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC6910.1277935182239845No Hit
AATATACATTTACACCAAGATATTATCAAAAAATGTGAACAGTTCGTAGG6810.12594411853912219No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA6720.12427965882274614No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT6700.12390977888577367No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC6670.123354958980315No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6460.11947121964210417No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6430.11891639973664549No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA6320.11688206008329696No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT6300.1165121801463245No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6260.11577242027237958No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT6250.11558748030389336No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA6200.1146627804614622No Hit
ACCATATACTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACC6180.11429290052448976No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC6100.11281338077659991No Hit
ATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCATCTCT6070.11225856087114122No Hit
TCCCGATACAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA5990.11077904112325139No Hit
ACTACATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATA5920.10948446134384779No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT5910.10929952137536156No Hit
AATGTGAACAGTTCGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTG5780.10689530178504057No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5760.10652542184806811No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG5730.10597060194260943No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5650.10449108219471959No Hit
CCAAGATATTATCAAAAAATGTGAACAGTTCGTAGGCCCACTCACAGTTA5620.10393626228926091No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5560.10282662247834352No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5520.1020868626043986No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5470.10116216276196746No Hit
CACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGG5460.10097722279348124No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5460.10097722279348124No Hit
TCTACGCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA5450.100792282824995No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT5450.100792282824995No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5410.10005252295105008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)