FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540716
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA37570.6948194616027638No Hit
ATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAG25630.47400113923020587No Hit
CATTTACACCAAGATATTATCAAAAAATGTGAACAGTTCGTAGGCCCACT25030.4629047411210321No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT22840.4224028880225479No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC22540.41685468896796096No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16330.30200696853801257No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT15680.2899858705864076No Hit
GCCACAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA14660.27112199380081226No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14530.2687177742104913No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGTTGACACACT13940.25780631606980375No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTCTAAATTTTAT13460.24892919758246473No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT12710.23505869994599754No Hit
GTATCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC12630.2335791801981077No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12390.2291406209544382No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG12220.2259966414901723No Hit
GTTCGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTA12060.22303760199439263No Hit
GTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCC11970.22137314227801655No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAAAAC11940.22081832237255788No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGT11230.20768758461003559No Hit
GTGTTGATTCTCTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAA11050.20435866517728346No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT10990.20324902536636608No Hit
CAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTAT10960.2026942054609074No Hit
ATGTCATGGGTCATTGCCACAAGAACACATCATACAAAAAATCAAAGAAT10840.20047492583907264No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG10360.19159780735173362No Hit
GTATCTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA9940.183830328675312No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACCTT9760.18050140924255986No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTCCTAAT9470.17513815015645923No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT9470.17513815015645923No Hit
CCCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCATC9030.16700079154306513No Hit
CACCAAGATATTATCAAAAAATGTGAACAGTTCGTAGGCCCACTCACAGT8810.16293211223636808No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG8710.16108271255150577No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT8600.15904837289815726No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT8600.15904837289815726No Hit
CTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTT8560.15830861302421234No Hit
GATTATGCCTGCTAGGTTTTATCCAAAGGTTACCAAATATTTGCCATTGG8560.15830861302421234No Hit
GAAAAGAAGATTGCAATTGATTATGCCTGCTAGGTTTTATCCAAAGGTTA8550.1581236730557261No Hit
TCGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGCAATTGATTATG8490.15701403324480873No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8480.15682909327632252No Hit
CCATATACTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACCA8470.15664415330783626No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT8270.15294535393811168No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7510.13888991633315825No Hit
ATCTCTTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGT7260.1342664171210025No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG7240.13389653718403005No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTA7220.1335266572470576No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7170.13260195740462646No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT7140.13204713749916777No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7140.13204713749916777No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC7040.1301977378143055No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCTCTTTTC7010.12964291790884677No Hit
TTCTATTACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6970.12890315803490188No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT6770.1252043586651773No Hit
AATATACATTTACACCAAGATATTATCAAAAAATGTGAACAGTTCGTAGG6700.12390977888577367No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC6610.12224531916939761No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6510.12039591948453533No Hit
CCATAACATCCAATGACATAGCCCATAAAATTTAGAGAGTAACCCCATCT6480.11984109957907663No Hit
GTTTTATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAAC6460.11947121964210417No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAGAGATGGGGTTACT6310.11669712011481072No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA6130.11336820068205859No Hit
CTCCGAAAGACACCAAATATTCTATTACTGTTTCTCTTCCAAAAGTGAGA6120.11318326071357238No Hit
GTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTTGTATGATGTGTT6110.11299832074508614No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6010.11114892106022385No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATTA6000.11096398109173762No Hit
AATGTGAACAGTTCGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTG5930.10966940131233402No Hit
ATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG5840.10800494159595796No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACTAGACACTATT5840.10800494159595796No Hit
CCAAGATATTATCAAAAAATGTGAACAGTTCGTAGGCCCACTCACAGTTA5840.10800494159595796No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5830.10782000162747174No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5810.10745012169049928No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC5800.10726518172201303No Hit
ACCATATACTTGGGAACAAAAGCTACAGCATGGGGCAGAATCTTTCCACC5770.10671036181655436No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5690.10523084206866451No Hit
GTGAAAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA5590.10338144238380223No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGATTAAAGA5530.10227180257288485No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT5520.1020868626043986No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT5500.10171698266742614No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCT5460.10097722279348124No Hit
ACTACATGTTCTGGATAATAAGGTTTAATACCCTTATCCAATGGCAAATA5410.10005252295105008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)