FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488672

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488672
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495556
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT18260.36847500585201265No Hit
GCCTAATCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTT16390.33073961368644517No Hit
GTTTTTATAGTGGGACACTTCAAACATTTTTCAGAGATTCTCTTGGGGTT14520.2930042215208776No Hit
CATATAAGGACAAAGAAATCCAAGCAGCAAGAAGCTGGAGGTATACAAGA14100.2845288927991993No Hit
ATTTTAAGCTGGTGCTGGACCTGGCAAAGATTTAAAATCATCTGTTTTTA14090.28432709925820693No Hit
GCCTAGTCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTT13780.2780714994874444No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA12610.2544616551913406No Hit
GTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11200.22600876591142072No Hit
CTATAAAAACAGATGATTTTAAATCTTTGCCAGGTCCAGCACCAGCTTAA11000.22197289509157392No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT10940.22076213384561988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10370.20925990200905648No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10280.20744376014012544No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT9990.20159174745134756No Hit
GTCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTTCAACT9890.1995738120414242No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9860.19896843141844717No Hit
GGATCATATTGTACAAAAAATCAAACAATGTTTTCGAAAACTTCCTGTCA9750.19674870246753143No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG9720.1961433218445544No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9510.19190565748371527No Hit
GTGCTGGACCTGGCAAAGATTTAAAATCATCTGTTTTTATAGTGGGACAC9170.18504467708997568No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT9160.18484288354898337No Hit
CTCCAAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8960.18080701272913657No Hit
TCCTAGGGGCTTCCTAAGGATTTAGAAGCCATCATTTGAAATAGAGACTT8490.17132271630249657No Hit
GTATACAAGAGCTTCCTAGGGGCTTCCTAAGGATTTAGAAGCCATCATTT8480.17112092276150426No Hit
GTATATGGACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8050.16244380049883364No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGAGTTT8020.16183841987585662No Hit
ATACAAGAGCTTCCTAGGGGCTTCCTAAGGATTTAGAAGCCATCATTTGA7570.15275771053120132No Hit
GTGCTGGTGGCAATGTAGTATCTTCTTTGGTCCTTGGCCAATGGGGTCAC7570.15275771053120132No Hit
AGTCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTTCAAC7440.1501343944983009No Hit
GTAGTATCTTCTTTGGTCCTTGGCCAATGGGGTCACATATAAGGACAAAG7380.14892363325234687No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT7180.14488776243250007No Hit
GTTCTATTTTAAGCTGGTGCTGGACCTGGCAAAGATTTAAAATCATCTGT7180.14488776243250007No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG6900.13923754328471455No Hit
GATTTAAAATCATCTGTTTTTATAGTGGGACACTTCAAACATTTTTCAGA6860.1384303691207452No Hit
ATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6650.13419270475990605No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT6600.13318373705494435No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTATCTGATCAGG6540.1319729758089903No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT6400.12914786623509755No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTATC6330.12773531144815117No Hit
TTCTTCTACTGTCCCTATTTTTAATCCTGTATGGCAAACTCCTTCTTTTC6310.12733172436616647No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6250.12612096312021245No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT6230.12571737603822777No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA6180.12470840833326607No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG6110.1232958535463197No Hit
TTATTATCCTGATCAGATAGTTAATCATTACTTTCAAACCCGACATTATT6060.12228688584135798No Hit
GGGTAAAGGTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5910.11925998272647288No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5850.11804922148051886No Hit
TATAAGGACAAAGAAATCCAAGCAGCAAGAAGCTGGAGGTATACAAGAGC5830.11764563439853418No Hit
CTTCAAACATTTTTCAGAGATTCTCTTGGGGTTCCGCCACCCGTACTTCC5670.11441693774265674No Hit
CCCCAAGAGAATCTCTGAAAAATGTTTGAAGTGTCCCACTATAAAAACAG5620.11340797003769502No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5490.1107846540047946No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA5440.10977568629983292No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAA5390.10876671859487122No Hit
GAGGAGAATCAAGGGAATAAGAAAGATTTTACTTCAACTAGTGACTGATG5330.10755595734891718No Hit
GGAAAATACAGGCCCCTGACTCTGGGATCTTGCAGAGCTTGGTGGAAAGC5270.10634519610296313No Hit
CTTATCTATCTGCCTCAATGGAAGTACGGGTGGCGGAACCCCAAGAGAAT5150.10392367361105506No Hit
GATCAGGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTG5140.10372188007006272No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)