FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences495556
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT16100.3248876009976673No Hit
GCCTAATCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTT15970.32226428496476683No Hit
GTTTTTATAGTGGGACACTTCAAACATTTTTCAGAGATTCTCTTGGGGTT14380.2901791119469848No Hit
ATTTTAAGCTGGTGCTGGACCTGGCAAAGATTTAAAATCATCTGTTTTTA14190.2863450346681303No Hit
CATATAAGGACAAAGAAATCCAAGCAGCAAGAAGCTGGAGGTATACAAGA12890.2601118743391262No Hit
GCCTAGTCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTT12750.2572867647652334No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATACCCCATGAA12130.2447755652237083No Hit
GTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA11180.225605178829436No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAGAGGCAAATATTTAGTGCTGTT10920.2203585467636352No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10350.2088563149270718No Hit
CTATAAAAACAGATGATTTTAAATCTTTGCCAGGTCCAGCACCAGCTTAA10100.2038114764022633No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA10070.20320609577928628No Hit
GTGCTGGACCTGGCAAAGATTTAAAATCATCTGTTTTTATAGTGGGACAC9590.193520005811654No Hit
CCATTTACACAATGTGGTTACCCTGCATTAATGCCTTTGTATGCATGTAT9470.1910984833197459No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAGAG8990.18141239335211357No Hit
GGATCATATTGTACAAAAAATCAAACAATGTTTTCGAAAACTTCCTGTCA8900.17959625148318253No Hit
GTCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTTCAACT8860.17878907731921317No Hit
CTCCAAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8710.17576217420432808No Hit
GTATATGGACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG8660.17475320649936638No Hit
GTATACAAGAGCTTCCTAGGGGCTTCCTAAGGATTTAGAAGCCATCATTT8280.16708505194165746No Hit
TCCTAGGGGCTTCCTAAGGATTTAGAAGCCATCATTTGAAATAGAGACTT8270.16688325840066512No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCCCTAAATTTCAT8220.16587429069570342No Hit
CCTCCAGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8180.16506711653173406No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7930.16002227800692556No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA7520.15174874282623962No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGAGTTT7380.14892363325234687No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTATCTGATCAGG7340.1481164590883775No Hit
ATACAAGAGCTTCCTAGGGGCTTCCTAAGGATTTAGAAGCCATCATTTGA7290.1471074913834158No Hit
GTAGTATCTTCTTTGGTCCTTGGCCAATGGGGTCACATATAAGGACAAAG7120.14367700118654603No Hit
AGTCATGGGAGAGAGGAGAATCAAGGGAATAAGAAAGATTTTACTTCAAC7070.14266803348158433No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG7030.14186085931761497No Hit
GATTTAAAATCATCTGTTTTTATAGTGGGACACTTCAAACATTTTTCAGA6810.13742140141578346No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT6770.13661422725181413No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT6750.13621064016982945No Hit
GTGCTGGTGGCAATGTAGTATCTTCTTTGGTCCTTGGCCAATGGGGTCAC6750.13621064016982945No Hit
GTATACATGCATACAAAGGCATTAATGCAGGGTAACCACATTGTGTAAAT6540.1319729758089903No Hit
GTTCTATTTTAAGCTGGTGCTGGACCTGGCAAAGATTTAAAATCATCTGT6470.13056042102204393No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA6460.1303586274810516No Hit
TTCTTCTACTGTCCCTATTTTTAATCCTGTATGGCAAACTCCTTCTTTTC6460.1303586274810516No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATACC6450.13015683394005925No Hit
ATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAATTAATTTCAGTCTT6250.12612096312021245No Hit
CCCCAAGAGAATCTCTGAAAAATGTTTGAAGTGTCCCACTATAAAAACAG5890.11885639564448822No Hit
TTATTATCCTGATCAGATAGTTAATCATTACTTTCAAACCCGACATTATT5850.11804922148051886No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTATC5830.11764563439853418No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT5810.11724204731654948No Hit
CTTCAAACATTTTTCAGAGATTCTCTTGGGGTTCCGCCACCCGTACTTCC5650.11401335066067206No Hit
TATAAGGACAAAGAAATCCAAGCAGCAAGAAGCTGGAGGTATACAAGAGC5610.1132061764967027No Hit
GTACGGGACCCTGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG5560.11219720879174098No Hit
CTTATCTATCTGCCTCAATGGAAGTACGGGTGGCGGAACCCCAAGAGAAT5390.10876671859487122No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAGTACCACATC5340.1077577508899095No Hit
GTCTAGAGGCAAATATTTAGTGCTGTTAGGATAAAACCTAGCAGGCATAA5340.1077577508899095No Hit
GGGTAAAGGTCCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5200.10493264131601675No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT5200.10493264131601675No Hit
CCTGTATGGCAAACTCCTTCTTTTCCTAAAATTCATTTACATGAAGACAT5040.10170394466013932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)