FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454883
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA33030.726120782706762No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19820.4357164369739032No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12300.2703992015529268No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT10490.23060874994229286No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT10280.22599217820846237No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10120.22247479022078206No Hit
GTTTATAAGAATACCAGTCATCTTGGATTGAAGCCCACCTTCATTTTAAC9910.2178582184869516No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG9570.21038376901313083No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8390.18444303260398828No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8340.18334384885783817No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC8190.18004629761938784No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA8020.17630907288247746No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT7970.17520988913632737No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7940.17455037888863728No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC7670.1686147866594267No Hit
GTCAATGAGAGAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCC7580.1666362559163565No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT7430.16333870467790618No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT7390.1624593576809861No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT7290.1602609901886859No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT7150.15718327569946558No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT7080.15564441845485544No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC7080.15564441845485544No Hit
CACTAATATGGGCCTAAAGTTCAGGCAATTATTGTGGTTTCACATTTCTT6440.14157486650413403No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6420.14113519300567398No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT6070.13344090678262321No Hit
GACATATCCCATGAAATGTAAAGAGTAACCCCATCTTTTTGTTTTGTTAG5910.12992351879494288No Hit
CTCTACGACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5900.12970368204571286No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5750.12640613080726254No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5570.12244906932112215No Hit
AATATACATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGG5500.12091021207651198No Hit
CTACGAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC5430.11937135483190184No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5410.11893168133344177No Hit
CTCACAGTCAATGAGAGAAGAAGACTGCAATTGATTATGCCTGCTAGGTT5410.11893168133344177No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC5310.11673331384114155No Hit
GGATATGTCATTGGCAGTTATGGGTCATTGCCGCAAGATCATATCATACA5280.1160738035934515No Hit
GCTTTATTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCT5230.1149746198473014No Hit
GAGAAGAAGACTGCAATTGATTATGCCTGCTAGGTTTTATCCAAATGTTA5200.11431510959961132No Hit
GATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTGG5160.11343576260269125No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG5100.1121167421073111No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC5050.11101755836116099No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG5010.11013821136424091No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT4900.10772000712271068No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT4890.10750017037348064No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT4800.10552163963041046No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4680.10288359863965019No Hit
GGTTCGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4650.10222408839196014No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT4640.10200425164273012No Hit
CTTATAAACAGGTGAAATTTGGAGACAGATAGACATGCAGCCAGAGGGGA4560.10024555764888993No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)