FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences742275
Sequences flagged as poor quality0
Sequence length35-151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA88421.191202721363376No Hit
AATATACACTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGG44790.6034151763160553No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAAC38210.5147687851537502No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT34220.4610151224276717No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT32320.4354181401771581No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT31410.42315853288875416No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG30310.4083392273752989No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT29910.4029503890067697No Hit
CACTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT25430.3425953992792429No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT23260.31336095112997203No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT22270.30002357616786235No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC21280.2866862012057526No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA21200.2856084335320468No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT20530.27658212926476033No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAA20520.2764474083055471No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN20010.26957663938567245No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTCTAAATTTCAT19430.2617628237513051No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACA19210.2587989626486141No Hit
ATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT19080.2570475901788421No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT18670.25152403085109964No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT18370.2474824020747028No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT17960.24195884274696033No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTA17710.2385908187666296No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT17700.23845609780741636No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC17450.23508807382708563No Hit
GTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGG15970.21514937186352767No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG14950.20140783402377826No Hit
CTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTGTACCTGTC13920.1875315752248156No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC13860.18672324946953622No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13700.18456771412212455No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA13650.1838941093260584No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC13600.18322050452999225No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGA13570.18281634165235255No Hit
CTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACACTTACACC13500.18187329493785995No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA13030.17554140985483818No Hit
GTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACA12930.17419420026270588No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTCTTCTACTG12230.1647637331177798No Hit
GCAATTAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGC12040.16220403489272844No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11910.16045266242295644No Hit
GTTTTATCCAAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAAC11730.15802768515711832No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT11370.15317773062544204No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11330.15263884678858913No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT11300.15223468391094946No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC11050.1488666599306187No Hit
ATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAG11040.1487319389714055No Hit
CTCTATAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGT10780.14522919403186152No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC10740.14469031019500858No Hit
AATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTAG10690.14401670539894246No Hit
TTAATACCCTTATCTAATGGCAAATATTTGGTAACATTTGGATAAAACCT10600.1428042167660234No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGGCTTTATTC10490.14132228621467785No Hit
TTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACCAGACATTATT10460.14091812333703815No Hit
CTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT10020.13499040113165606No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA9980.13445151729480315No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9960.13418207537637666No Hit
GGCATAATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTAC9860.13283486578424436No Hit
CCTACAAATTGTTCACATTTTTTGATGATGTCTTGGTGTAAGTGTATATT9860.13283486578424436No Hit
CTTTTCCTAATATACACTTACACCAAGACATCATCAAAAAATGTGAACAA9850.13270014482503117No Hit
ATTAATTGCAGTCTTCGTTTCTCATTGACTGTGAGTGGACCTACAAATTG9750.13135293523289887No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA9620.12960156276312687No Hit
GTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGACAGGTA9390.1265029807012226No Hit
ACTTTACAGGGCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGG9390.1265029807012226No Hit
CTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTA9310.12542521302751675No Hit
TAATTATGCCTGCTAGGTTTTATCCAAATGTTACCAAATATTTGCCATTA9290.1251557711090903No Hit
CCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGAT9020.12151830521033309No Hit
ATATACACTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGT9010.12138358425111988No Hit
GAGTATGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCT9000.12124886329190664No Hit
AACTAGATGTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAAT8980.12097942137348017No Hit
CTCTTTTCCTAATATACACTTACACCAAGACATCATCAAAAAATGTGAAC8980.12097942137348017No Hit
GTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8780.11828500218921559No Hit
GGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATTAATTG8610.11599474588259069No Hit
CTCTAAATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCACAA8440.1137044895759658No Hit
GTGTTGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA8320.11208783806540702No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC8260.11127951231012766No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTACT8260.11127951231012766No Hit
TCCTAATATACACTTACACCAAGACATCATCAAAAAATGTGAACAATTTG8260.11127951231012766No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT8170.11006702367720858No Hit
GTCTTGGTGTAAGTGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT8050.10845037216664982No Hit
CTAATATACACTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTA8050.10845037216664982No Hit
CCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACT8050.10845037216664982No Hit
GAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCT8030.10818093024822337No Hit
ATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCATTCAGAAA8010.1079114883297969No Hit
ATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAA7900.10642955777845138No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG7700.10373513859418679No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAG7640.10292681283890742No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC7630.10279209187969418No Hit
ATATTTGCCATTAGATAAGGGTATTAAACCTTATTATCCAGAACATCTAG7580.10211848708362803No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7540.10157960324677512No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCATCATACTGCACTCAGGCA7520.10131016132834865No Hit
CTCACAGTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTT7450.10036711461385604No Hit
ATATTTGGTAACATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTC7440.10023239365464282No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)