FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378703
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGA21450.5664069204627373No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17900.4726659149782283No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14840.39186380884228533No Hit
GTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT13630.3599126492264387No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10870.2870323182018627No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10670.2817511347942847No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG10360.27356530051253886No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCCCTAAATTTTAT9040.23870949002252426No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8810.2326361291038096No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCGCTGACACACT8540.2255065315035793No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG8470.22365811731092705No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT8440.22286593979979033No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA8160.21547228302918117No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7860.20755050791781424No Hit
CCCTTATCCAATGGCAAATATTTGGTGACATTGGGATAAAACCTAGCAGG7720.20385367953250966No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7690.20306150202137296No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTTCACATTTCTT7670.20253338368061516No Hit
CTTACATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCTTG7600.20068496948796286No Hit
CTCTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGT7560.19962873280644725No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCAGAGAGTCCAA7420.19593190442114267No Hit
CTTATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTT7320.1932913127173537No Hit
CTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCACTTTTCCTAAT7300.1927631943765959No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATTGGGATAAAAC7140.18853824765053354No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACT7140.18853824765053354No Hit
AGCTTGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA7030.18563359677636565No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA6990.18457736009485007No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTTAGT6920.18272894590219776No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGGACAACAGAGT6880.18167270922068218No Hit
GAGAAACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACC6860.18114459087992438No Hit
GTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCATACTG6710.17718370332424088No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6650.1755993483019675No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6490.17137440157590514No Hit
GTTTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGAAATTGATTA6280.16582915899794826No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6260.16530104065719048No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6210.16398074480529595No Hit
CATCAAGACATTATCAAAAAATGTGAACAGTTTGTAGGCCCACTCACAGT6150.16239638978302257No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAATCTCCTGA5900.15579491052355013No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACT5830.15394649633089783No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5740.15156996379748774No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5580.14734501707142536No Hit
TAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGAAGT5560.14681689873066756No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5550.14655283956028867No Hit
CTCTTGGACTCTCTGCAATGTCAACGACCGACCTTGAGGCATACTTCAAA5450.14391224785649967No Hit
ACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTT5320.140479478641574No Hit
CTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCTTCAC5160.13625453191551162No Hit
CTTTCCAATCAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTG5060.13361394021172265No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATT4990.13176552601907035No Hit
GTGTCTCTCAAAGGTGGAGACAGTGGGGTAGGCTGCCTTCCTGACTGGCG4960.13097334850793366No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4950.13070928933755474No Hit
ATATAATATACCCGCCTGCCATAGAGTGTGTAAATAGTGTCTAGTTTGGA4890.12912493431528135No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4890.12912493431528135No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC4860.12833275680414466No Hit
CTGTAACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAA4830.12754057929300797No Hit
GCTTTGGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4810.1270124609522502No Hit
CTCCGAAAGACACCAAATACTCTATAACTGTTTCTCTTCCAAAAGTGAGA4760.1256921651003557No Hit
CAGTTAATGAGAAAAGAAGATTGAAATTGATTATGCCTGCTAGGTTTTAT4730.124899987589219No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA4690.1238437509077034No Hit
TTGTAGGCCCACTCACAGTTAATGAGAAAAGAAGATTGAAATTGATTATG4650.1227875142261878No Hit
GTACGACATCTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGA4620.12199533671505111No Hit
GTATGGATCGGCAGAGGAGCCATAAAGGTTCCACGCATGCGCTGATGGCC4590.12120315920391442No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCACTTTTC4490.11856256750012543No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4490.11856256750012543No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG4440.11724227164823092No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAG4440.11724227164823092No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTAACCAATTTTCTT4440.11724227164823092No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC4410.11645009413709423No Hit
GCTCTGTATCGGGATGCCTTAGAATCTCCTGAGCATTGTTCACCTCACCA4360.11512979828519974No Hit
GTCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG4360.11512979828519974No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGATTTGGTGGGCGTTC4350.11486573911482084No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4310.11380950243330525No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4260.11248920658141076No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT4220.11143296989989517No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC4220.11143296989989517No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA4190.11064079238875847No Hit
CCTCAAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4190.11064079238875847No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCTGCAATGTCAACGACCGACCT4160.10984861487762178No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4120.10879237819610618No Hit
ACCCATAACATCCAATGACATAGCCCATAAAATTTAGGGAGTAACCCCAT4110.1085283190257273No Hit
TCTAGGGACCTAGTAGTCAGTTATGTCAACACTAATATGGGCCTAAAGTT4100.10826425985534839No Hit
GCTTTGGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGG4100.10826425985534839No Hit
GTGTGTAAATAGTGTCTAGTTTGGAAGTAATGATTAACTAGATGTTCTGG4060.1072080231738328No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGTCT4060.1072080231738328No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATTCG4030.1064158456626961No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC4020.1061517864923172No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC3990.1053596089811805No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3980.10509554981080162No Hit
GTTATGTCAACACTAATATGGGCCTAAAGTTCAGGCAACTCTTGTGGTTT3970.10483149064042271No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT3970.10483149064042271No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA3950.10430337229966491No Hit
ACGACATCTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAAT3910.10324713561814933No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3890.10271901727739152No Hit
CCTACAAACTGTTCACATTTTTTGATAATGTCTTGATGTAAATGTATATT3850.10166278059587591No Hit
GTTTTATCCCAATGTCACCAAATATTTGCCATTGGATAAGGGTATTAAAC3830.10113466225511812No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTCATTGGAAAACACCCA3830.10113466225511812No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3830.10113466225511812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)