FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488680

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488680
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466171
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA18990.40736124726763356No Hit
GTCTCTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT15320.3286347713607239No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA12260.26299362251191083No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12060.2587033513453218No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT11940.2561291886453683No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT11510.2469051056372018No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10080.21622966679608985No Hit
GTCTGTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT9710.20829266513790004No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9330.20014114992138077No Hit
CATTAGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA9280.19906858212973352No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8940.19177512114653206No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTAGGATAAAACCTAGCAGG8500.18233652458003607No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC8390.1799768754384121No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA8050.17268341445521063No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC7760.16646252126365646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG7710.1653899534720092No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT7550.16195773653873793No Hit
CCCACAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG7520.16131419586374957No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA7110.15251913997224195No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7060.15144657218059468No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7040.15101754506393578No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTAGGATAAAAC6910.14822886880565286No Hit
ATCCTAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6820.14629824678068776No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6480.13900478579748635No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC6240.13385646039757942No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT6160.1321403519309438No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6120.13128229769762598No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6070.1302097299059787No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT6050.1297807027893198No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6040.12956618923099036No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5860.12570494518106015No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC5680.12184370113113No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGACGAAACGG5670.12162918757280053No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT5620.12055661978115327No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5520.11841148419785873No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5460.117124402847882No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5430.11648086217289363No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5410.11605183505623473No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5360.11497926726458745No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5340.11455024014792854No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5320.11412121303126964No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5290.11347767235628128No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5280.11326315879795182No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT5240.11240510456463401No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT5220.1119760774479751No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5210.11176156388964566No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5100.10940191474802165No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT5090.10918740118969218No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5080.10897288763136274No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG4980.1068277520480682No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT4920.10554067069809148No Hit
CTGTGATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT4890.10489713002310311No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4890.10489713002310311No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4880.10468261646477364No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4850.1040390757897853No Hit
GATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGAGTAACTCC4840.10382456223145584No Hit
GTATGGATCGGCAGAGGAGCCGAGAAGGTTCCACGCATGCGCTGATGGCC4830.10361004867312638No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4740.10167942664816129No Hit
GGCAACTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACG4690.10060685885651403No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4670.10017783173985513No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)