FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488681

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488681
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences466171
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16550.35501993903524676No Hit
GTCTCTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT15880.3406475306271733No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11980.25698724287868613No Hit
CCTCCAGCTTATAGACCACCTAATGCCCCTATCTTATCAACACTTCCGGA11690.250766349687132No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT10680.22910048029585708No Hit
CATTTACACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACT10600.22738437182922144No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10100.21665869391274875No Hit
GTCTGTGGGTATACATTTAAACCCTAACAAAACTAAAAGATGGGGTTATT9870.2117248820711713No Hit
CATTAGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA9270.19885406857140406No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8710.1868413093049546No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC8510.18255103813836554No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCACAGAGAGTCCAA8100.17375598224685793No Hit
CCCTTATCTAATGGCAAATATTTGGTAACATTAGGATAAAACCTAGCAGG8050.17268341445521063No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTAGGATAAAAC7900.1694657110802688No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG7650.16410287212203248No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTATTCTTTAAATTTCAT7320.15702392469716048No Hit
CTCTATGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA6990.1499449772722885No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6770.1452256789890405No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC6620.1420079756140987No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6430.13793221800583905No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTGATGTCAACGACCGACCT6320.13557256886421507No Hit
CCCACAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG6150.13192583837261435No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGCTGGATCTTC6100.13085327058096707No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6050.1297807027893198No Hit
ATCCTAATGTTACCAAATATTTGCCATTAGATAAGGGTATTAAACCTTAT6040.12956618923099036No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5980.12827910788101363No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5870.12591945873938962No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG5830.1250614045060718No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG5820.12484689094774234No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATT5700.1222727282477889No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5670.12162918757280053No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5670.12162918757280053No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5540.11884051131451764No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5510.11819697063952928No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5420.11626634861456417No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5420.11626634861456417No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5300.11369218591461072No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5290.11347767235628128No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5250.11261961812296346No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5250.11261961812296346No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5240.11240510456463401No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5090.10918740118969218No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5090.10918740118969218No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT4980.1068277520480682No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT4950.10618421137307983No Hit
CACCAAGACATCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGT4940.1059696978147504No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG4900.10511164358143256No Hit
CTCTCGTCCCCTTCTCCGTCTGCCGTTTCGTCCGACCACGGGGCGCACCT4890.10489713002310311No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4870.1044681029064442No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACATTAGGATAAAA4800.10296650799813802No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4790.10275199443980858No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4710.10103588597317294No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)