FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544742
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG37180.6825249384112112No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30410.5582459219226716No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT29360.53897074211278No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT26760.49124172544066735No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT18780.3447503588854907No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16240.2981227810596576No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTCTGACATACT15860.2911470016998873No Hit
ACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACAT15020.275726857851974No Hit
CTACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC13620.25002661810545174No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA13520.24819088669498587No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13430.24653872842556662No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13330.2447029970151007No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC13000.23864508336056334No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA12910.23699292509114406No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12610.23148573085974644No Hit
GGTTTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG12540.23020071887242033No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA12370.22707997547462838No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC11570.2123941241909014No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA11240.206336210536364No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC11180.20523477169008447No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA10700.1964232609198483No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10130.18595959188019284No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG9830.1804523976487952No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9490.17421091085321125No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC9470.17384376457111808No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG9370.1720080331606522No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAAC9370.1720080331606522No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCGGAACATGCAGTTAATCA9120.16741870463448752No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA9070.16650083892925457No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAA8640.15860719386425134No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8490.15585359674855254No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8490.15585359674855254No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG8470.15548645046645934No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG8150.14961210995296856No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8100.1486942442477356No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC8080.14832709796564245No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7800.14318705001633802No Hit
GGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAAT7770.14263633059319825No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7730.1419020380290119No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7700.14135131860587213No Hit
CAATAGGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGT7660.1406170260416858No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7650.1404334529006392No Hit
ACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTGAGG7620.13988273347749944No Hit
GGGTAAAGGTTTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7470.13712913636180063No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA7470.13712913636180063No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7350.1349262586692416No Hit
ATCTAAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7340.134742685528195No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG7340.134742685528195No Hit
ATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT7290.13382481982296207No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7110.13052050328412348No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAAATAGA7090.13015335700203032No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA6980.12813405245051787No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA6940.1273997598863315No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA6770.12427901648853953No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCT6760.12409544334749294No Hit
CCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGACA6720.12336115078330659No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6590.12097469994970095No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6550.12024040738551461No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6520.11968968796237485No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6490.11913896853923509No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG6440.11822110283400215No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA6390.11730323712876921No Hit
GTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA6220.11418249373097723No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6200.11381534744888407No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACAT6180.11344820116679089No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6140.11271390860260454No Hit
GCTTGTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGT5980.10977673834585915No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA5820.10683956808911375No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5810.10665599494806716No Hit
GAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAA5760.10573812924283423No Hit
GTGTAGTTTCTCTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAA5730.10518740981969446No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC5680.10426954411446152No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA5590.10261738584504224No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGGAT5570.10225023956294907No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5560.10206666642190247No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAAT5520.10133237385771612No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAAATAGACCTAT5510.10114880071666954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)