FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544742
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG41180.755954194829846No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30240.5551251785248796No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT26370.48408237293985046No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT25640.47068153364344956No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT20140.36971630606782663No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16960.31134004721501185No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA14730.2704032367616229No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC14550.26709892022278436No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA13710.25167877637487107No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG13560.24892517925917224No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTCTGACATACT13130.241031534194169No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12720.23350503541125892No Hit
ACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACAT12720.23350503541125892No Hit
CTACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC12290.22561139034625566No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC11800.21661630643497287No Hit
GGTTTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG11790.21643273329392632No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCC11600.21294484361404115No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA11370.20872266136996964No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11210.20578549111322425No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA10250.18816246957275187No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA9880.18137026335402814No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9870.18118669021298156No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT9690.177882373674143No Hit
ACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA9360.1718244600196056No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAA8890.16319652239041602No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC8890.16319652239041602No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC8820.1619115104030899No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC8790.16136079097995012No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGAAGTTTTCGAAAAC8530.15658788931273887No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG8470.15548645046645934No Hit
GTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATA8400.15420143847913326No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8350.1532835727739003No Hit
ACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATGTGAGG8250.15144784136343442No Hit
ATATTGTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAAATAGA8210.1507135487992481No Hit
GGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAAT8200.1505299756582015No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG8100.1486942442477356No Hit
ATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGA7980.14649136655517658No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7950.14594064713203683No Hit
ATCTAAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7840.14392134258052436No Hit
ATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGTACAATATGTT7810.14337062315738458No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA7690.14116774546482555No Hit
CCCTTAGACAAAGGCATTAAACCTTATTATCCGGAACATGCAGTTAATCA7620.13988273347749944No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7610.13969916033645285No Hit
ATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAG7530.13823057520808016No Hit
CAATAGGTCTATTTACAGGAAGTTTTCGAAAACATTGCTTGAGTTTTAGT7520.13804700206703358No Hit
GGGTAAAGGTTTAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7470.13712913636180063No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7440.13657841693866088No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7380.13547697809238135No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG7170.131621942130403No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTACTAAAA7120.13070407642517007No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7080.12996978386098373No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6960.12776690616842468No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG6890.12648189418109856No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACAT6830.12538045533481904No Hit
GTTTTAGTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA6830.12538045533481904No Hit
GTACTAAAACTCAAGCAATGTTTTCGAAAACTTCCTGTAAATAGACCTAT6710.12317757764226No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6670.12244328507807366No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA6650.12207613879598048No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6590.12097469994970095No Hit
CAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGA6590.12097469994970095No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6560.1204239805265612No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA6470.11877182225714192No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGGAT6380.11711966398772262No Hit
GTCTTATATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6290.11546750571830335No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6210.11399892058993064No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA5860.1075738606533001No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5780.10610527552492739No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5770.10592170238388082No Hit
CTTTACCACAGGAACATATTGTACTAAAACTCAAGCAATGTTTTCGAAAA5720.10500383667864786No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG5680.10426954411446152No Hit
TATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAATGAAAAAA5650.10371882469132176No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5650.10371882469132176No Hit
CCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGACA5640.10353525155027518No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5610.1029845321271354No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5540.1016995201398093No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5530.10151594699876272No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT5480.10059808129352978No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5470.10041450815248319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)