FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473533
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16820.35520227734920273No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13350.2819233295250806No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG12630.2667184757978852No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12380.26143901269816466No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT11820.2496130153547905No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG10750.2270169132879863No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10000.21117852398882445No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9890.20885556022494736No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT9800.2069549535090479No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9650.2037872756492156No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9430.19914134812146142No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGATACTCCCTTAACTTCAT9260.19555131321365143No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8940.18879360044600904No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8920.1883712433980314No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8670.18309178029831077No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA7820.1651416057592607No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7390.15606092922774126No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7310.15437150103583067No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG7150.15099264465200948No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6550.13832193321268No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTT6510.1374772191167247No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6470.13663250502076943No Hit
GCCTAGCAGCCATGGAAACGAGGTGTATTTCCGAGAGAGCACAACAGAGT6440.13599896944880294No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGCG6420.1355766124008253No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6290.13283129158897058No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT6240.13177539896902646No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6060.1279741855372276No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6030.12734064996526112No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTAAT6020.12712947144127232No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6010.1269182929172835No Hit
GCACTAAGGCAAGCCATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5960.12586240029733936No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5950.12565122177335056No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5920.12501768620138407No Hit
GTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC5750.12142765129357405No Hit
GCTTACTACTAATTCCCTGGATGCCGGGTCTTCCAAATTACTTCCCACCC5750.12142765129357405No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5710.12058293719761876No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5700.12037175867362992No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5600.11825997343374167No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5530.11678172376581991No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5510.11635936671784226No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5490.11593700966986462No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGATTA5470.11551465262188695No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5420.11445876000194283No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5410.11424758147795402No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5350.11298051033402107No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5200.10981283247418872No Hit
GTCCTGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5100.10770104723430045No Hit
CCTTTACCCCGTTGCTCGGCAACGATCAGGTCTTTGCCAAGTGTTTGCTG5050.10664515461435635No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5050.10664515461435635No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT5030.1062227975663787No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC4880.10305511970654634No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4850.10242158413457986No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4800.10136569151463573No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4790.1011545129906469No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4760.10052097741868042No Hit
AGTTAAGGGAGTATCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA4750.1003097988946916No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA4740.10009862037070277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)