FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505910
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28980.5728291593366409No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT16570.3275286118084244No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT14290.28246130734715663No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA13530.267438872526734No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12820.2534047557866024No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG12700.251032792393904No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT12320.24352157498369276No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA11590.2290921310114447No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10790.21327904172678938No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10460.20675614239686899No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10240.20240754284358875No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9980.19726828882607578No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCACTGAATTTCAT9720.19212903480856278No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9540.18857108971951533No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG9430.18639678994287523No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8950.176908936372082No Hit
CCATTTACACAATGTGGTTATCCTGCATTAATGCCTTTGTATGCATGTAT8810.1741416457472673No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG8360.16524678302464865No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8170.161491174319543No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8140.16089818347136842No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT8050.1591192109268447No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG7990.15793322923049552No Hit
CTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC7790.1539799569093317No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTGGGATAAAACCTAGCAGGCATAA7790.1539799569093317No Hit
GTATACATGCATACAAAGGCATTAATGCAGGATAACCACATTGTGTAAAT7780.1537822932932735No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT7690.15200332074874978No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT7190.14212013994584016No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG6840.13520191338380344No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6820.13480658615168706No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT6790.13421359530351248No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAGTACCACATC6450.12749303235753395No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT6040.11938882409914806No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTACTCTTCTACTG5990.1184005060188571No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCA5980.11820284240279892No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA5920.11701686070644977No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA5810.11484256092980966No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTATACC5800.11464489731375146No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG5660.11187760668893677No Hit
TCCTCAGGCCATGCAATGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG5650.11167994307287857No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5630.11128461584076219No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5490.1085173252159475No Hit
GTAGAAGAGTAAAGTCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5480.1083196615998893No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5460.10792433436777292No Hit
CTCATAAGGTGGGAAATTTTACTGGACTTTACTCTTCTACTGTCCCTATC5280.10436638927872548No Hit
ATACTAACATGGGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCT5210.10298274396631811No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5210.10298274396631811No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG5190.10258741673420174No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5180.10238975311814354No Hit
CTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCT5170.10219208950208536No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT5090.1006107805736198No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC5070.10021545334150342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)