FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505910
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28900.5712478504081754No Hit
CTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGTTT15510.3065762685062561No Hit
GGATAATAAGGTTTAATCCCTTTGTCTAAAGGCAAATATTTTGTGCTGTT13370.2642762546698029No Hit
ATATGATCCTGTGGCAATGTACCCCAACTTCCAATTACATAACCCATGAA13340.2636832638216284No Hit
GCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGTAAATGAAAAAAG13240.26170662766104646No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12150.24016129351070348No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11870.2346267122610741No Hit
CCTATTAGCAATGTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTT11560.22849914016327016No Hit
GTGTAAATGGAGCGGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT11540.22810381293115375No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA10910.21565100511948765No Hit
GGATCATATTGTACAAAAAATCAAACACTGTTTTAGAAAACTTCCTGTCA10690.2113024055662074No Hit
CTATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATA10400.20557016070051987No Hit
GTATATGAACCTTTACCCCGTTGCCCGGCAACGGCCTGGTCTGTGCCAAG10350.20458184262022888No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTATTCACTGAATTTCAT9700.1917337075764464No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9350.18481548101440967No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA9070.17928089976478032No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGATCACCCGTG8980.17750192722025657No Hit
CCATTTACACAATGTGGTTATCCTGCATTAATGCCTTTGTATGCATGTAT8940.1767112727560238No Hit
CTCTTCTACTGTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTC8840.1747346365954419No Hit
TTATTATCCTGATCATGTAGTTAATCATTACTTTCAAACCCGACATTATT8590.16979304619398708No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT8520.1684094008815797No Hit
CTCCGAAAGAGACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGG8420.1664327647209978No Hit
GTATACATGCATACAAAGGCATTAATGCAGGATAACCACATTGTGTAAAT8220.16247949239983395No Hit
CATGAAGACATTGCTAATAGGTGTCAGCAATTTGTAGGCCCTCTCACTGT8110.16030519262319384No Hit
CTCTTATATAGAATCCCAGCCTTCCAAAGAGTATGTAAATAATGTCGGGT7860.15536360222173903No Hit
GTCTAAAGGCAAATATTTTGTGCTGTTGGGATAAAACCTAGCAGGCATAA7670.1516079935166334No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCCGTCCG7530.1488407028918187No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAGTACCACATC7490.14805004842758593No Hit
GGGCTGTATCGTCCCCTTCTCCGTCTGCCGTACCGTCCGACCACGGGGCG7410.1464687394991204No Hit
CTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCATTTACATG6600.13045798659840682No Hit
GTACCACATCATCCATATAGCTGAAAGCCAAACAGTGGGGGAAAGCCCTA6410.1267023778933012No Hit
ATGTGGTACTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTATACC6400.12650471427724297No Hit
ATACAGCCCCAAGCGGCCTCGCGAGGGGTCGTCCGCGGGATTCAGCGCCG6300.12452807811666107No Hit
GTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACATGATCAGG5980.11820284240279892No Hit
TTCTAAAACAGTGTTTGATTTTTTGTACAATATGATCCTGTGGCAATGTA5940.11741218793856614No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGACTTTACTCTTCTACTG5910.11681919709039157No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGCTCACCTCATCA5840.11543555177798423No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGAGACCAAATACTCAAGGA5830.11523788816192604No Hit
ATATAGAGCTGAGGCTGTATCTAGTAGATCCCGGACGGAAGGAAAGAAGT5820.11504022454586783No Hit
GGCCTAAAGATCAGGCAATTATTGTGGTTTCATATTTCTTGCCTTACTTT5720.11306358838528592No Hit
GTCTTCATGTAAATGAATTTTAGGAAAAGAAGGGGTTTGCCATTCAGGAT5630.11128461584076219No Hit
TCCTCAGGCCATGCAATGGAATTCCACAGCTTTCCACCAAGCTCTGCAAG5550.10970330691229666No Hit
GTCCCTATCTTTAATCCTGAATGGCAAACCCCTTCTTTTCCTAAAATTCA5520.10911031606412208No Hit
CTCAAGGACAGTTTCTCTTCCAAAAGTAAGGCAAGAAATATGAAACCACA5510.10891265244806388No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5440.10752900713565654No Hit
ATATAAGAGGGAAACTACACGTAGCGCCTCATTTTGCGGGTCACCATATT5400.10673835267142376No Hit
GTAGAAGAGTAAAGTCCAGTAAAATTTCCCACCTTATGAGTCCAAGGAAT5340.10555237097507461No Hit
GTTTTAGAAAACTTCCTGTCAATCGACCTATTGATTGGAAAGTATGTCAG5270.10416872566266727No Hit
CTTTAGGCCCATGTTAGTATTAACATAATTGACTACTAGATCCCTGGATG5200.10278508035025993No Hit
CTCATAAGGTGGGAAATTTTACTGGACTTTACTCTTCTACTGTCCCTATC5190.10258741673420174No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG5190.10258741673420174No Hit
TCCAAAGAGTATGTAAATAATGTCGGGTTTGAAAGTAATGATTAACTACA5080.10041311695756162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)