FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294647
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA15740.5341985494506987No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15430.5236774852620254No Hit
GTATACATGTATACAAAGGCAGCAAAGCAGGATATCCACATTGTGTAAAA14300.4853265093484746No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA14000.4751448343271779No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC13980.4744660559924248No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13720.4656419376406344No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC12430.42186073504905874No Hit
GTTACATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAGCATCTTGAG11550.39199448831992184No Hit
GGGGCTACTCCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACT10870.36891602493831605No Hit
GGCAAAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGT10740.3645039657624208No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAATTTTCTAGGGGGAACTCCCGTG9560.32445604401198724No Hit
GGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG9130.30986230981479534No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC8400.28508690059630676No Hit
GCACAGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG8360.28372934392680055No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8170.27728094974664597No Hit
CTAGAGGACCTGCCTCGTTGTCTAACAACAGTAGTTTCCGGAAGTGTTGA7790.26438416138633686No Hit
CATGTATACAAAGGCAGCAAAGCAGGATATCCACATTGTGTAAAAGGGGC7770.26370538305158375No Hit
GAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGA7700.26132965887994786No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAGTTTTCTAGGGGGAACTACCGTG7490.2542024863650402No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG7490.2542024863650402No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7360.249790427189145No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGCTAATGAATT7300.24775409218488562No Hit
TGGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCATTAC7210.24469958967849667No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA6860.2328209688203172No Hit
GTATACATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATA6850.23248157965294064No Hit
GAACTCCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC6830.2318028013181875No Hit
GTCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG6800.23078463381605788No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG6720.22806952047704543No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC6520.22128173712951427No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT6440.21856662379050185No Hit
GGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTAAACAATATC6430.21822723462312532No Hit
CGGTAGTTCCCCCTAGAAAACTGAGAGAAGTCAACCACGAGTCTAGACTC6260.21245761877772387No Hit
GTCCTGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC6190.21008189460608798No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG5770.19582754957627263No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5490.18632465288972905No Hit
GCAAAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTT5470.18564587455497597No Hit
GTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG5410.1836095395507166No Hit
CCGTATGGTGGAGTGAACCCTGGCCCGAATGCTCCCACTCCTACCTGATT5380.18259137204858694No Hit
GTAAAGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTT5310.18021564787695107No Hit
TACGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAG5280.17919748037482142No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC5220.17716114537056205No Hit
GGCCCCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTC5030.1707127511904075No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCACAG4980.16901580535352473No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCTGTG4960.16833702701877162No Hit
GTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA4930.16731885951664197No Hit
CCATGTAACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4750.16120985450386394No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC4710.15985229783435773No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4580.15544023865846252No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4530.15374329282157972No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAAATGGGAGTAGGCTGTCT4520.15340390365420317No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4500.15272512531945007No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4480.15204634698469693No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC4470.15170695781732038No Hit
ATCCTACCCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT4420.15001001198043762No Hit
GTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGCC4400.14933123364568449No Hit
GGTCTTTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC4310.1462767311392955No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT4250.14424039613503617No Hit
GTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAGCATCTTGAG4170.14152528279602372No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4140.14050711529389406No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4110.13948894779176438No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4110.13948894779176438No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA3910.13270116444423327No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3880.13168299694210361No Hit
GTGTTGGGGTGAGCTAATGAATTTGGCCACCTGGGTGGGAAGTAATTTGG3830.12998605110522082No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTCAGGCA3830.12998605110522082No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3820.12964666193784427No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3800.12896788360309117No Hit
ACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGA3780.12828910526833803No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3770.12794971610096148No Hit
GACTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG3760.12761032693358493No Hit
ATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT3740.12693154859883182No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3700.12557399192932558No Hit
CTTTTGGGGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC3690.12523460276194903No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC3690.12523460276194903No Hit
TACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACACGC3660.12421643525981937No Hit
CTTTTACACAATGTGGATATCCTGCTTTGCTGCCTTTGTATACATGTATA3620.12285887859031315No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA3600.12218010025556005No Hit
GGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAAT3600.12218010025556005No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATG3570.12116193275343039No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG3570.12116193275343039No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3560.12082254358605382No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAAGAGTCCTCTTATGT3550.12048315441867727No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3540.1201437652513007No Hit
CCCCAAAAGACCGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGCTCCCA3530.11980437608392416No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC3520.1194649869165476No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG3510.11912559774917104No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA3500.11878620858179448No Hit
ACTACACGCAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA3490.11844681941441794No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCCATACG3440.11674987357753516No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3430.1164104844101586No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA3430.1164104844101586No Hit
CCCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3430.1164104844101586No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCT3410.11573170607540549No Hit
AACCACAATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCT3410.11573170607540549No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACAACGAGGC3370.11437414940589927No Hit
GTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACACGCAGCGCCTCAT3350.11369537107114615No Hit
ATGTGGATATCCTGCTTTGCTGCCTTTGTATACATGTATACAATCCAAGC3350.11369537107114615No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3340.11335598190376958No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3340.11335598190376958No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3330.11301659273639304No Hit
TTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA3330.11301659273639304No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC3300.11199842523426336No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAGGAGGGA3290.11165903606688682No Hit
GGATGGGGCTTGGCCATAGGCCACCAGCGCATGCGTGGAACCTTTGTGGC3290.11165903606688682No Hit
GTTCTTGAGTATTTGGTGTCTTTTGGGGTGTGGATTCGCACTCCTACCGC3280.11131964689951027No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGCTAATGAATTTG3270.1109802577321337No Hit
CTCCCACTCCTACCTGATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTG3240.10996209023000404No Hit
GGGTTCACTCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA3220.10928331189525092No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC3220.10928331189525092No Hit
CTCCCGTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAA3210.10894392272787437No Hit
GCAGAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTT3210.10894392272787437No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3200.10860453356049782No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3190.10826514439312125No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCA3160.1072469768909916No Hit
CTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACACGCAGCGCC3160.1072469768909916No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3150.10690758772361503No Hit
CTTATATAAAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3140.10656819855623849No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3100.10521064188673226No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG3080.10453186355197915No Hit
CTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACACGCAGCGCCTC3070.10419247438460258No Hit
ATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTA3040.10317430688247292No Hit
GTCTTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA3040.10317430688247292No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3040.10317430688247292No Hit
GTGGTGGAGTGAACCCTGGCCCGAATGCTCCCACTCCTACCTGATTTGCC3020.10249552854771982No Hit
CATAATGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGC3020.10249552854771982No Hit
GGAACATATTGTACACAAAATCAAGCAATGTTTTCGAAAATTGCCTGTAA3000.1018167502129667No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2990.10147736104559014No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG2980.1011379718782136No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG2960.10045919354346047No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT2960.10045919354346047No Hit
GGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG2960.10045919354346047No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC2950.10011980437608392No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)