FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488693

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488693
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences294647
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATGTATACAAAGGCAGCAAAGCAGGATATCCACATTGTGTAAAA13340.45274514928032533No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13060.4432422525937817No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA12680.43034546423347264No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12550.4259334050575774No Hit
CATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATAAGGCC12120.4113396708603855No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA12110.41100028169300895No Hit
GTTACATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAGCATCTTGAG10370.35194656656948825No Hit
GGGGCTACTCCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACT9630.32683176818362314No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAATTTTCTAGGGGGAACTCCCGTG9540.32377726567723414No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC9260.31427436899069056No Hit
GGTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC8480.2878020139353192No Hit
GGCAAAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGT8440.286444457265813No Hit
GTCTAGACTCGTGGTTGACTTCTCTCAGTTTTCTAGGGGGAACTACCGTG7940.2694749988969852No Hit
GCACAGGACCCTGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7560.25657821053667607No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7430.25216615136078085No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG7430.25216615136078085No Hit
CATGTATACAAAGGCAGCAAAGCAGGATATCCACATTGTGTAAAAGGGGC7150.2426632546742373No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT7110.2413056980047311No Hit
GAACTCCCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC7060.23960875216784833No Hit
GGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGTCTCAATCG7020.2382511954983421No Hit
GTATACATGTATACAATCCAAGCAGGCTTTCACTTTCTCGCCAACTTATA6990.2372330279962124No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6990.2372330279962124No Hit
GAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGA6770.2297664663139282No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA6340.21517273211673632No Hit
CTAGAGGACCTGCCTCGTTGTCTAACAACAGTAGTTTCCGGAAGTGTTGA6190.21008189460608798No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGCTAATGAATT6090.2066880029323224No Hit
TGGACAAAGGCATTAAACCGTATTATCCTGAACATGCAGTTAATCATTAC5860.19888205208266163No Hit
GTCATTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG5860.19888205208266163No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACTTTCCACCAAACTCTGCAAG5790.19650632791102574No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG5610.19039732289824773No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5220.17716114537056205No Hit
GTCCTGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5210.1768217562031855No Hit
GGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTAAACAATATC5160.17512481036630273No Hit
GTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAA5140.17444603203154962No Hit
GGCCCCTCACCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTC5060.17173091869253718No Hit
CCCTTAACTTCATGGGATATATAATTGGAAGTTGGGGTACTTTACCACAG5020.17037336202303094No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC4980.16901580535352473No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC4920.16697947034926539No Hit
GTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGG4870.16528252451238262No Hit
CGGTAGTTCCCCCTAGAAAACTGAGAGAAGTCAACCACGAGTCTAGACTC4860.16494313534500604No Hit
GCAAAGACCTGACCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTT4830.16392496784287638No Hit
TACGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAG4580.15544023865846252No Hit
CCGTATGGTGGAGTGAACCCTGGCCCGAATGCTCCCACTCCTACCTGATT4540.15408268198895628No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4460.15136756864994383No Hit
GTAAAGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTT4460.15136756864994383No Hit
GTCTCTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGCC4420.15001001198043762No Hit
TTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACAC4350.14763428780880172No Hit
GTATTATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATT4230.14356161780028306No Hit
TATCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4200.14254345029815338No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4150.14084650446127062No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4140.14050711529389406No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4110.13948894779176438No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTCAGGCA4030.13677383445275193No Hit
ATCCTACCCTTACCAAATATTTGCCCTTGGACAAAGGCATTAAACCGTAT3990.13541627778324572No Hit
CCATGTAACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3980.13507688861586917No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3980.13507688861586917No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCTGTG3870.13134360777472706No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAAATGGGAGTAGGCTGTCT3820.12964666193784427No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTATATATCCCATGAA3800.12896788360309117No Hit
GTTATATGGATGATGTGGTATTGGGGGCGAAGTCTGTACAGCATCTTGAG3770.12794971610096148No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3700.12557399192932558No Hit
ATCCTGAACATGCAGTTAATCATTACTTCCAAACTAGGCATTATTTACAT3680.1248952135945725No Hit
CTTATAAGGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCC3680.1248952135945725No Hit
CTTTTGGGGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC3670.12455582442719593No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGGTGGCC3610.12251948942293658No Hit
ATGTGGATATCCTGCTTTGCTGCCTTTGTATACATGTATACAATCCAAGC3590.12184071108818349No Hit
GGTCTTTGCCAAGTGTTTGCTGACGCAACCCCCACTGGATGGGGCTTGGC3570.12116193275343039No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG3540.1201437652513007No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3530.11980437608392416No Hit
GTGTTGGGGTGAGCTAATGAATTTGGCCACCTGGGTGGGAAGTAATTTGG3490.11844681941441794No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA3480.11810743024704137No Hit
CTTTTACACAATGTGGATATCCTGCTTTGCTGCCTTTGTATACATGTATA3420.11607109524278203No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3410.11573170607540549No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG3390.11505292774065237No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3370.11437414940589927No Hit
CCCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3350.11369537107114615No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3290.11165903606688682No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3270.1109802577321337No Hit
CCCCAAAAGACCGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGCTCCCA3270.1109802577321337No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA3260.11064086856475713No Hit
GCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTC3210.10894392272787437No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3200.10860453356049782No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC3160.1072469768909916No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3150.10690758772361503No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC3140.10656819855623849No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTGTGGAGTTCCACTGCATG3130.10622880938886194No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT3120.10588942022148537No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT3080.10453186355197915No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT3080.10453186355197915No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGA3060.10385308521722604No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA3060.10385308521722604No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCAGGGTCCT3040.10317430688247292No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3040.10317430688247292No Hit
GGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAAT3020.10249552854771982No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3010.10215613938034326No Hit
GGGTTCACTCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA2980.1011379718782136No Hit
ACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGA2960.10045919354346047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)