FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295144
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGCAGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGA12040.40793646491204294No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11380.38557449922749576No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9020.3056135310221451No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8640.2927384598704361No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8630.2923996422085491No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8490.28765619494212996No Hit
CTCCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7490.25377442875342204No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6900.23378418670208442No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6810.23073482774510068No Hit
CTCCATCTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6700.22700783346434283No Hit
GTTGTGAGTAAGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG6620.2242972921692462No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATCAAAGCAGGATATCCACAT6500.22023148022660127No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG6300.21345512698885968No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6270.21243867400319844No Hit
GACCTTTAACCTAACCTCCTCCCCTAACTCCTCCCACTCATTAAACACAC5870.19888596752771526No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5820.1971918792182799No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5660.19177079662808663No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5530.1873661670235546No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC5480.18567207871411923No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5450.18465562572845798No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5330.18058981378581304No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5200.176185184181281No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5060.1714417369148619No Hit
CCCTTTTTTCAAAGCCAACTCAGACAATCCAGATTGGGACCTCAACCCGC4900.16602065432466864No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4820.163310113029572No Hit
GGATAATACGGTTTAATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4800.16263247770579783No Hit
CAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA4770.16161602472013661No Hit
AACGCGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGC4680.1585666657631529No Hit
CAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4640.15721139511560459No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC4540.1538232184967338No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4500.1524679478491855No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4450.1507738595397501No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4440.150435041877863No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4310.14603041227333097No Hit
GATCATTAACCTAACCTCCTCCCCTAACTCCTCCCACTCATTAAACACAC4260.1443363239638956No Hit
GCGTAAAAGGGGCGGCAAACCCTAAAAGACCCACAATTCGTTGACATACT4190.14196460033068603No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4120.13959287669747647No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4080.13823760604992816No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT4080.13823760604992816No Hit
CTCAAACAATCCAGATTGGGACCTCAACCCGCACAAGGACAACTGGCCGG4030.13654351774049278No Hit
CCCTAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3990.13518824709294444No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3970.13451061176917029No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3920.1328165234597349No Hit
CTTTTAGGGTTTGCCGCCCCTTTTACGCAATGTGGATATCCTGCTTTGAT3910.13247770579784782No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3850.13044479982652535No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3820.1294283468408641No Hit
CAGGAGGAGTGCGAATCCACACCCCAAAAGACACCAAATATTCAAGAACA3790.12841189385520288No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3760.12739544086954163No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3750.12705662320765457No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT3700.1253625348982192No Hit
TAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTGCAGT3670.12434608191255794No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3670.12434608191255794No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG3650.12366844658878379No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3640.12332962892689671No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3630.12299081126500963No Hit
CCCCTAACTCCTCCCACTCATTAAACACACAGTCTTTGAAGTATGCCTCA3610.12231317594123547No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3600.1219743582793484No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA3590.12163554061746132No Hit
GCGGCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCAC3560.12061908763180008No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3550.120280269969913No Hit
ACTACATACTCTGGATAATACGGTTTAATCCCTTTATCTAAGGGCAAATA3540.11994145230802593No Hit
CGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCTCACTG3540.11994145230802593No Hit
CCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG3500.1185861816604776No Hit
CATTAACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCC3490.11824736399859052No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTAAAA3480.11790854633670345No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT3450.11689209335104221No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3420.11587564036538098No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3420.11587564036538098No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3410.1155368227034939No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG3370.11418155205594557No Hit
AAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTCAGT3340.11316509907028434No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3340.11316509907028434No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3300.11180982842273603No Hit
CATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCC3290.11147101076084895No Hit
CTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT3270.11079337543707479No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA3260.11045455777518771No Hit
ACAAGTTGGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGAC3230.10943810478952648No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT3230.10943810478952648No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC3230.10943810478952648No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3180.10774401648009108No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3150.10672756349442984No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3140.10638874583254276No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG3120.10571111050876861No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA3110.10537229284688153No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3110.10537229284688153No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3090.10469465752310737No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3090.10469465752310737No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG3050.10333938687555905No Hit
AAAACTCAGACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGA3050.10333938687555905No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC3040.10300056921367197No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3000.10164529856612366No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG2970.10062884558046242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)